Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (23): 3767-3772.doi: 10.12307/2021.052

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Selection of modeling methods for acute compressive spinal cord injury: a network Meta-analysis

Xu Weilong1, Zuo Yuan2, Xin Daqi2, He Chenyang1, Zhao Peng1, Shi Ming1, Zhou Boyuan1, Liu Yating1, Zhao Yan2   

  1. 1Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia Autonomous Region, China; 2The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia Autonomous Region, China
  • Received:2020-04-09 Revised:2020-04-16 Accepted:2020-09-11 Online:2021-08-18 Published:2021-02-24
  • Contact: Zhao Yan, Professor, Master’s supervisor, The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia Autonomous Region, China
  • About author:Xu Weilong, Master candidate, Inner Mongolia Medical University, Hohhot 010000, Inner Mongolia Autonomous Region, China
  • Supported by:
    the Scientific Research Project of Inner Mongolia Autonomous Region, No. 2019GG157 (to ZY)

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Abstract: OBJECTIVE: Generating an acute compressive spinal cord injury model with an aneurysm clip is a simple and effective modeling method. However, to create the animal model of acute compressive spinal cord injury, many experimental studies have utilized diverse modeling methods according to different closure force aneurysm clips and duration, including aneurysm clipping with 30 g compression force to compress the spinal cord for 60 seconds (30 g, 60 s); aneurysm clipping with 70 g compression force to compress the spinal cord for 30 seconds (70 g, 30 s); aneurysm clipping with 70 g force to compress the spinal cord for 60 seconds (70 g, 60 s); compressing the spinal cord to 1/2 of its diameter for 30 seconds by aneurysm clips (1/2, 30 s). The present study aimed to assess whether there is a significant difference among these modeling methods, and to determine the method that is most suitable to establish the clamp-type spinal cord injury model.
METHODS: A literature search of PubMed, Embase, CNKI, VIP, and WanFang databases was conducted for relevant studies from their inception dates to October 1, 2019. The included literatures were initially screened according to the inclusion and exclusion criteria, and data extraction and quality evaluation were performed subsequently. The paired Meta-analysis, subgroup analysis, and network Meta-analysis were conducted to evaluate the effects of different modeling methods on the limb function of spinal cord injury rats.
RESULTS: Fourteen articles with a total of 714 Sprague-Dawley rats were included in the study. All the included articles were scored 6-8 for quality evaluation. The Basso, Beattie and Bresnahan (BBB) scores of the four modeling methods in the 1st week after modeling ranked as follows: sham operation group > 30 g, 60 s group > 1/2, 30 s group > 70 g, 60 s group > 70 g, 30 s group. The BBB score in the 30 g, 60 s group was significantly higher than that in the 1/2, 30 s group, 70 g, 60 s group and 70 g, 30 s group at 1 week postoperatively (P < 0.05). Subgroup analyses suggested an association of locomotor recovery of spinal cord injury with the sex of rats. Differences in circadian activities of rats also affected the BBB scores.
CONCLUSION: The four modeling methods described in the study can be used to create the animal model of acute compressive spinal cord injury. Comparison of the four modeling methods has revealed that the best way to create a clamp-type spinal cord injury model is to compress the spinal cord for 60-second aneurysm clipping with a closing force of 30 g. It is recommended to use female rats for modeling, but further research and verification are needed.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: spinal cord injury, model, aneurysm, rat, locomotor recovery, sex, network Meta-analysis, evidence-based medicine

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