Structure analysis of platelet-rich fibrin derived from two centrifugation procedures

doi:10.3969/j.issn.2095-4344.3189

Abstract

Abstract: BACKGROUND: Platelet-rich fibrin has been widely used in clinic because of its characteristics, but the structure and composition of fibrin produced by different centrifugation procedures will be different, which will affect the clinical effect.
OBJECTIVE: To compare the histological characteristics of platelet-rich fibrin prepared by the different centrifugation procedures.
METHODS: Venous blood samples from 40 voluntary patients were divided into two groups (n=20). In group A, modified platelet-rich fibrin was obtained by low-speed centrifugation (1 500 r/min centrifugation for 14 minutes). In group B, traditional platelet-rich fibrin was obtained by medium-speed centrifugation
(3 000 r/min for 10 minutes). Hematoxylin-eosin staining, immunohistochemistry, scanning electron microscopy, and transmission electron microscope were used to analyze the difference of leukocytes and fibrin matrix in two groups of platelet-rich fibrin.
RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining: Both groups contained more leucocytes, mostly concentrated in the middle layer and biased towards the end layer of erythrocytes. Gel layer was mainly composed of fibrin matrix, and the leucocytes were coagulated in the advanced platelet-rich fibrin. The glue layer was scattered and evenly distributed. (2) Immunohistochemistry: The two groups contained a large number of leucocytes in the middle layer, including monocytes and neutrophils. The proportion of neutrophils in the modified platelet-rich fibrin was greater than that of the leukocyte-rich platelet fibers (P < 0.05). There was no significant difference in the ratio of monocytes between the two groups (P > 0.05). (3) Scanning electron microscopy: The leucocytes of the two groups were distributed slightly widely. The numbers of leucocytes and platelet in the advanced platelet-rich fibrin were slightly higher than those in leucocyte- and platelet-rich fibrin. The fiber matrix cords were slightly tightly arranged in the modified platelet-rich fibrin. (4) Transmission electron microscopy: The two groups of intermediate layers gathered a large number of platelets and many neutrophils and monocytes. The protein matrix was densely arranged, among which the fiber matrix in the modified platelet-rich fibrin was slightly denser than that in leucocyte- and platelet-rich fibrin. (5) The results showed that the modified platelet-rich fibrin may be better than leucocyte- and platelet-rich fibrin due to the difference in the number of leucocytes. The advanced platelet-rich fibrin may play a more active role in promoting tissue healing.

Key words: materials, platelet-rich fibrin, improvement, centrifugation, tissue engineering, inflammation, neutrophils, monocytes, platelets

CLC Number:

Bi Qingwei, Liu Chengpu, Li Yan, Zhao Wenwen, Han Mei. Structure analysis of platelet-rich fibrin derived from two centrifugation procedures[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(22): 3534-3539.

Figures/Tables 5

References

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