Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (50): 9341-.doi: 10.3969/j.issn.1673-8225.2010.50.008

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Cultivation and biological differences of fibroblasts derived from human normal skin and keloid 
 

Guo Li-li, Liu Lin-bo, Chen Min-jing   

  1. Department of Plastic Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou   450052, Henan Province, China
  • Online:2010-12-10 Published:2010-12-10
  • Contact: Liu Lin-bo, Professor, Chief physician, Doctoral supervisor, Department of Plastic Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China liulinbo@zzu.edu.cn
  • About author:Guo Li-li★, Master, Attending physician, Department of Plastic Surgery, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052, Henan Province, China

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Abstract:

BACKGROUND: Fibroblasts are the main effector cells during wound healing, thus, cultivation of fibroblasts derived from keloid provides a basis for the in vitro keloid study.
OBJECTIVE: To observe the biological difference of the fibroblasts derived from normal skin and keloid.
METHODS: The fibroblasts from keloid and normal skin were primary cultured, subcultured, froze, and resuscitated in vitro. The proliferation and differences of normal skin and keloid fibroblasts were observed.
RESULTS AND CONCLUSION: The fibroblasts isolated from normal skin and keloid exhibited similar morphology and growth rates. The frozen cells were resuscitated successfully after cryopreservated in liquid nitrogen. There were significances of survival rate prior to and after cryopreservation. The results demonstrated that, there are no obviously differences of fibroblasts isolated from normal skin and keloid, both of which can resuscitate successfully.

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