Chinese Journal of Tissue Engineering Research ›› 2010, Vol. 14 ›› Issue (50): 9346-.doi: 10.3969/j.issn.1673-8225.2010.50.009

Previous Articles     Next Articles

Effects of insulin-like growth factor-1 and fibroblast growth factor-7 on growth of dermal papilla cells

Zhang Guo-qiang1, Cheng Yi1, Li Ling2, Gao Shun-qiang1   

  1. 1 Department of Dermatology, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China; 2 Department of Dermatology, First Central Hospital of Baoding, Baoding 071000, Hebei Province, China
  • Online:2010-12-10 Published:2010-12-10
  • Contact: Gao Shun-qiang, Chief physician, Professor, Department of Dermatology, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China gaoshunqiang@medmail.com.cn
  • About author:Zhang Guo-qiang★, Master, Attending physician, Department of Dermatology, Fourth Affiliated Hospital of Hebei Medical University, Shijiazhuang 050011, Hebei Province, China zgq810328@sina.com
  • Supported by:

    Chinese Medical Association-L’OREAL China Skin/Hair Grant, No. H2009030412*

PDF

714

Abstract:

BACKGROUND: Agglutinative growth of dermal papilla cells (DPCs) is related to their abilities to induce hair growth. Growth factors secreted by DPCs, such as insulin-like growth factor-1 (IGF-1) and fibroblast growth factor-7 (FGF-7), play an important role in DPCs growth. 
OBJECTIVE: To observe correlation between DPCs proliferation, growth characteristics and IGF-1 and FGF-7.
METHODS: Dermal papillas were isolated from human scalp hair follicles by two-step digestion method. The expressions of IGF-1, FGF-7 protein in different passages of cultured DPCs were detected by immunohistochemical method and flow cytometry. Effects of different concentration of IGF-1 (2.5-100 μg/L) on the cell proliferation of DPCs were measured by MTT.
RESULTS AND CONCLUSION: The result of immunohistochemistry showed that IGF-1 and FGF-7 were expressed in the cytoplasm, which decreased with time prolonged. The expression of IGF-1 in the 3rd generation DPCs was obviously greater than that of the 9th generation (P < 0.05). Compared with the control group, different concentrations of IGF-1 could significantly promote the proliferation of DPCs (P < 0.05), especially in the 2.5 μg/L. The change of the aggregative growth of DPCs may be related to the decrease of the expressions of IGF-1 and FGF-7. IGF-1 can stimulate the proliferation of DPCs significantly.

CLC Number: