Protective effects of sulforaphane in a H2O2 induced oxidative stress model of human hepatocytes

doi:10.3969/j.issn.2095-4344.2123

Abstract

Abstract:

BACKGROUND: Oxidative stress injury plays an important role in the development of chronic hepatitis, and sulforaphane is a natural product with good antioxidant activity.

OBJECTIVE: To investigate the protective effects of sulforaphane on oxidative stress injury of human hepatocytes (LO2) stimulated by H₂O₂, and to investigate its possible mechanism.

METHODS: LO2 cells were cultured in vitro, followed by 24-hour treatment with 100 μg/L H₂O₂ to induce the oxidative stress model of the cells. The cells were divided into five groups: control group, model group, sulforaphane treatment groups (low-, middle-, high-dose groups: H₂O₂+10, 20, and 40 μmol/L sulforaphane, respectively). Cell counting kit-8 was used to measure the relative survival rate of cells, and analyze the levels of oxidative stress products in cell homogenate. Western blot assay was used to determine the expression levels of

nuclear factor erythroid-2 related factor 2 (Nrf2), GCLC, NQO1 and heme oxygenase-1. The study protocol was in accordance with the related ethic requirements of Central Hospital of Enshi Autonomous Prefecture.

RESULTS AND CONCLUSION: H₂O₂ could induce oxidative stress injury in LO2 cells. Compared with the model group, sulforaphane at various concentrations could significantly induce the proliferation of LO2 cells, inhibit the production of malondialdehyde and nitric oxide, and promote the activity of superoxide dismutase (P < 0.05). Western blot results revealed that sulforaphane could induce the transfer of Nrf2 into the nucleus and significantly increase the expression of GCLC, NQO1, and heme oxygenase-1 proteins (P < 0.05). To conclude, sulforaphane can significantly relieve the oxidative stress injury of LO2 cells induced by H₂O₂ and its underlying mechanism may be related to the activation of Nrf2 pathway.

Key words: sulforaphane, human hepatocyte, oxidative stress, heme oxygenase-1, nuclear factor erythroid-2 related factor 2, protein

CLC Number:

Tan Jing, Yi Guodong. Protective effects of sulforaphane in a H2O2 induced oxidative stress model of human hepatocytes[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(31): 5052-5056.

Figures/Tables 4

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