Pulsed electromagnetic fields stimulation combined with
sclerostin antibody improves bone metabolism and bone microstructure in
postmenopausal osteoporosis rabbits

doi:10.3969/j.issn.2095-4344.2611

Abstract

Abstract:

BACKGROUND: Both pulsed electromagnetic fields (PEMF) stimulation and sclerostin antibody (Scl-Ab) have good effects on the bone metabolism of ovariectomized (OVX) New Zealand rabbits, but research on the combined intervention of PEMF and Scl-Ab in the OVX rabbits is rarely reported.

OBJECTIVE: To explore the effect of PEMF combined with Scl-Ab on postmenopausal osteoporosis and to explore the therapeutic value for osteoporosis.

METHODS: An animal model of postmenopausal osteoporosis was made in New Zealand white rabbits after ovariectomy. The experimental animals were randomly divided into OVX control group, PEMF group, Scl-Ab group and PEMF+Scl-Ab group, with 10 rats in each group. On the 1^st day after surgery, the PEMF group was given PEMF magnetic therapy once a day; the Scl-Ab group was given subcutaneous injection of Scl-Ab twice a week; the PEMF+Scl-Ab group received PEMF magnetic therapy once a day, five times a week, and Scl-Ab subcutaneous injections twice a week; the OVX group was injected subcutaneously with the same dose of normal saline twice a week for 8 weeks. After 8 weeks of treatment, bone metabolism index, bone mineral density, and MicroCT bone microstructure parameters were detected. All animal procedures were approved by the Department of Experimental Animal Science, Fudan University (approval No. 20171263A193).

RESULTS AND CONCLUSION: Bone mineral density was significantly decreased in the New Zealand white rabbits after 6 months of OVX, suggesting that the osteoporosis model was successfully established. Compared with the OVX group, the bone mineral density of the L₃ vertebral body in the PEMF group, the Scl-Ab group and the PEMF+Scl-Ab group increased significantly (P < 0.05). Compared with the OVX group, serum bone-specific alkaline phosphatase levels were significantly higher, and serum tartrate-resistant acid phosphatase 5b levels were significantly lower in the PEMF group, the Scl-Ab group and the PEMF+Scl-Ab group. The serum tartrate-resistant acid phosphatase 5b level in the PEMF+Scl-Ab group was significantly lower than that in the PEMF group and the Scl-Ab group. The bone metabolism index and bone microstructural parameters (bone volume fraction, trabecular thickness, trabecular number, and trabecular separation) of the PEMF+Scl-Ab group were significantly better than those of the PEMF group and the Scl-Ab group (all P < 0.05). These findings indicate that the combination of Scl-Ab and PEMF can enhance bone mineral density and improve bone metabolism and bone microstructure in postmenopausal osteoporosis New Zealand white rabbits.

Key words: pulsed electromagnetic field, sclerostin antibody, combination treatment, osteoporosis, bone mass, bone mineral density, rabbit

CLC Number:

Qian Guang, Yu Yueming, Dong Youhai, Hong Yang, Wang Minghai. Pulsed electromagnetic fields stimulation combined with sclerostin antibody improves bone metabolism and bone microstructure in postmenopausal osteoporosis rabbits[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(20): 3130-3134.

Figures/Tables 5

References

[1] GENANT HK, ENGELKE K, BOLOGNESE M, et al. Effects of Romosozumab Compared with Teriparatide on Bone Density and Mass at the Spine and Hip in Postmenopausal Women with Low Bone Mass.J Bone Miner Res.2017;32(1):181-187.
[2] LINDSAY R, KREGE J, MARIN F, et al. Teriparatide for osteoporosis: importance of the full course. Osteoporosis Int.2016; 27(8):2395-2410.
[3] KE H, RICHARDS W, LI X, et al. Sclerostin and Dickkopf-1 as therapeutic targets in bone disease.Endoer Rev.2012;33:747-783.
[4] HAY E, BOUAZIZ W, FUNCKBRENTANO T, et al. Sclerostin and Bone Aging: A Mini-Review.Gerontology.2016;62(6):618-623.
[5] MINISOLA S, CIPRIANI C, OCCHIUTO M, et al. New anabolic therapies for osteoporosis.Intern Emerg Med.2017;12:915-921.
[6] WALDORFF EI, ZHANG N, RYABY JT. Pulsed electromagnetic field applications: A corporate perspective.J Orthop Translat. 2017;9:60-68.
[7] ANTONINO C, SAVERIO L, FEDERICA B, et al. Pulsed electromagnetic fields modulate bone metabolism via RANKL/OPG and Wnt/β-catenin pathways in women with postmenopausal osteoporosis: A pilot study.Bone. 2018; 116: 42-46.
[8] 邱裕友,唐翠松,胡剑,等.催产素干预兔骨质疏松模型骨微结构变化规律的实验研究[J].同济大学学报(医学版), 2015,36(5):23-28.
[9] LI X, OMINSKY M, WARMINGTON K, et al. Sclerostin antibody treatment increases bone formation, bone mass, and bone strength in a rat model of postmenopausal osteoporosis.J Bone Miner Res.2009; 24:578-588.
[10] OMINSKY M, VLASSEROS F, JOLETTE J, et al. Two doses of sclerostin antibody in cynomolgus monkeys increases bone formation, bone mineral density, and bone strength.J Bone Miner Res.2010;25:948-959.
[11] XU XJ, SHEN L, YANG YP, et al. Serum sclerostin levels associated with lumbar spine bone mineral density and bone turnover markers in patients with postmenopansal osteoporosis.Chin Med J,2013;126(13): 2480-2484.
[12] SPATZ JM, WEIN MN, GOOI JH, et al. The Wnt inhibitor sclerostin is up-regulated by mechanical unloading in osteocytes in vitro.J Biol Chem.2015;290(27):16744-16758.
[13] MCCOLM J, HU L, WOMACK T, et al. Single-and multiple-dose randomized studies of blosozumab,a monoclonal antibody again stsclerostin in healthy postmenopausal women.J Bone Miner Res. 2014;29:935-943.
[14] MARKOV M. Pulsed electromagnetic field therapy history, state of the art and future. Environmentalist.2007; 27:465-475.
[15] 覃裕,邱冰,朱思刚,等.唑来膦酸盐联合脉冲电磁场治疗绝经后骨质疏松症的临床疗效分析[J].中国骨质疏松杂志, 2015;21(8):945-948.
[16] JUN Z, YUAN L, HAITAO X, et al. Effects of combined treatment with Ibandronate and Pulsed Electromagnetic Field on ovariectomy-induced osteoporosisin rats.Bioelectromagnetics.2017; 38:31-40.
[17] IKEDA KS, TAKESHITA S. The role of osteoclast differentiation and function in skeletal homeostasis. J Biochem.2016;159(1):1-8.