Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 6019-6024.doi: 10.3969/j.issn.2095-4344.2012.32.025

Previous Articles     Next Articles

Expression of neurotrophic factors in rats with intracerebral hemorrhage following transplantation of bone marrow stromal cells modified by glial cell line-derived neurotrphic factor gene

Deng Li, Chang Neng-bin, Xiong Huai-lin, Gao Xiao-qing, Yang Chao-xian   

  1. 1Department of Neurobiology, 2Department of Anatomy, Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Received:2012-02-20 Revised:2012-03-28 Online:2012-08-05 Published:2012-08-05
  • Contact: Yang Chao-xian, Professor, Department of Neurobiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China yangchaoxian2006@tom.com
  • About author:Deng Li★, Master, Lecturer, Department of Neurobiology, Luzhou Medical College, Luzhou 646000, Sichuan Province, China dy9611@163.com

Abstract:

BACKGROUND: Previous studies have demonstrated that cell transplantation combined with neurotrophic factor can promote neural function recovery after brain injury.
OBJECTIVE: To explore the effects of transplantation of bone marrow stromal cells (BMSCs) modified by glial cell line-derived neurotrphic factor (GDNF) gene on expression of neurotrophic factors in rats with intracerebral hemorrhage.
METHODS: Forty-eight rats were used to establish the models of intracerebral hemorrhage by injecting with collagenase and heparin into caudate nucleus through a stereotaxic apparatus. Then rats were randomly divided into BMSCs group, BMSCs/GDNF group and saline group and were stereotaxically injected with BMSCs, GDNF/BMSCs and saline respectively at the 3rd day after intracerebral hemorrhage induction.
RESULTS AND CONCLUSION: Compared with saline group and BMSCs group, neurological function recovery was better in the GDNF/BMSCs group (P < 0.05). At 1, 2 weeks after cell transplantation, the expression of neurotrophic factors in the BMSCs group and GDNF/BMSCs group was significantly increased than in the saline group (P < 0.05). Transplantation of BMSCs modified by GDNF gene provides better neuroprotective effects than transplantation of simple BMSCs in treatment of intracerebral hemorrhage.

CLC Number: