Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 6011-6013.doi: 10.3969/j.issn.2095-4344.2012.32.023

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Best storage time for red blood cells prior to freezing

Wang Chi-lin, Xie Yu-bin, Qiu Ming, Kuang Kai-qi, Zhong Dai-ming   

  1. Changsha Blood Center, Changsha 410001, Hunan Province, China
  • Received:2011-12-03 Revised:2012-02-24 Online:2012-08-05 Published:2012-08-05
  • About author:Wang Chi-lin, Chief technician, Changsha Blood Center, Changsha 410001, Hunan Province, China wangchilin6@sina.com

Abstract:

BACKGROUND: Appropriately extending the storage time of red blood cells prior to freezing and ensuring the quality of frozen red blood cells are beneficial for blood use in clinical emergency and can decrease blood costs.
OBJECTIVE: To appropriately extend the storage time at (4±2) ℃ for rare-type red blood cells prior to freezing, to meet the needs of emergency blood transfusion in patients with rare blood types, decrease blood costs, and to reduce the work intensity.
METHODS: A quality comparison was performed between red blood cells stored at (4±2) ℃ for 1-6 days and red blood cells stored at (4±2) ℃ for 7-12 days. According to the principle of blood preservative solution to protect red blood cells, the U.S. AABB related standards as well as the quality control results, the storage time for additive-containing red blood cells prior to freezing should be set within 14 days from the date of blood collection. Rh(D)-negative blood supplied by our center between 2001 and 2009 was statistically analyzed before and after taking this measure.
RESULTS AND CONCLUSION: Quality test results met the national standards and two sets of quality control data showed no significant statistical difference. The storage time for red blood cells with additives prior to freezing was set within 14 days after the date of blood collection. Although the annual blood supply of Rh(D)-negative blood of our center increased year by year, the supply of frozen red blood cells was not increased and its proportion in the total blood supply decreased yearly, while the proportion of blood supply for blood stored at (4±2) ℃ increased gradually. No clinical blood quality problems were found regarding the red blood cells which were stored at (4±2) ℃ for 14 days prior to freezing. Results showed that increasing the blood supply of Rh(D)-negative blood stored at (4±2) ℃ and decreasing the blood supply of frozen red blood cells can meet the needs of emergency blood transfusion of Rh D)-negative patients in time, lower the high blood costs for preparation of frozen red blood cells, and reduce the work intensity for staffs in preparing the frozen red blood cells.

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