Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (7): 1037-1045.doi: 10.3969/j.issn.2095-4344.2035
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Huang Cheng1, Liu Yuanbing1, Dai Yongping1, Wang Liangliang1, Cui Yihua1, Yang Jiandong2
Received:
2019-08-09
Revised:
2019-08-14
Accepted:
2019-09-17
Online:
2020-03-08
Published:
2020-01-19
Contact:
Yang Jiandong, Chief physician, Associate professor, Master’s supervisor, Department of Spine Surgery, Northern Jiangsu People’s Hospital, Yangzhou 225000, Jiangsu Province, China
About author:
Huang Cheng, Master, Physician, Department of Orthopedics, Rugao People’s Hospital, Rugao 226500, Jiangsu Province, China
Supported by:
CLC Number:
Huang Cheng, Liu Yuanbing, Dai Yongping, Wang Liangliang, Cui Yihua, Yang Jiandong. Transplantation of bone marrow mesenchymal stem cells overexpressing glial cell line derived neurotrophic factor gene for spinal cord injury[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(7): 1037-1045.
2.7 免疫组化检测脊髓损伤局部神经元特异性烯醇化酶、突触素Ⅰ及胶质纤维酸性蛋白表达 免疫组化染色结果显示BMSCs-GDNF移植治疗后4周脊髓损伤局部可见较多神经元特异性烯醇化酶阳性细胞分布,BMSCs移植组脊髓损伤局部亦可见神经元特异性烯醇化酶阳性细胞散在分布,但阳性细胞数较少,而PBS移植对照组未见明显阳性细胞。BMSCs-GDNF移植组与BMSCs移植组脊髓损伤局部可见棕褐色突触素Ⅰ蛋白表达,PBS移植组仅少许突触素Ⅰ蛋白表达,统计学分析比较各组神经元特异性烯醇化酶蛋白平均光密度值差异有显著性意义。另外,PBS移植组脊髓损伤局部可见棕褐色胶质纤维酸性蛋白阳性细胞大量分布,BMSCs移植组亦可见明显胶质纤维酸性蛋白阳性细胞分布,BMSCs-GDNF移植组脊髓损伤局部虽可见胶质纤维酸性蛋白表达但平均光密度值较PBS移植组、BMSCs移植组明显降低,差异有显著性意义,见图6,7。 "
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