Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (1): 1-6.doi: 10.3969/j.issn.2095-4344.2001

    Next Articles

Effects of vascular endothelial growth factor combined with platelet-derived growth factor-BB on the angiogenic and proliferative abilities of bone marrow mesenchymal stem cells

Sun An, Bi Xiangyu, Han Xiangzhen, Zhou Qiqi, He Huiyu   

  1. Department of Prosthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Received:2019-06-15 Revised:2019-06-18 Accepted:2019-07-18 Online:2020-01-08 Published:2019-12-10
  • Contact: He Huiyu, MD, Chief physician, Professor, Master’s supervisors, Doctoral supervisor, Department of Prosthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Sun An, Master candidate, Department of Prosthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:
    the National Natural Science Foundation of China, No. 81660177

PDF

191

Abstract:

BACKGROUND: Bone tissue engineering provides a new path to treat critical-sized bone defects. However, a stable osteogenesis and osseointegration can only be insured via formation of an intact vascular network, which can achieve satisfactory therapeutic effects. Thereafter, angiogenesis is a challenge and difficulty in bone tissue engineering.

OBJECTIVE: To investigate the effect of combined application of vascular endothelial growth factor (VEGF) and platelet-derived growth factor-BB (PDGF-BB) on proliferation and angiogenesis of bone marrow mesenchymal stem cells.

METHODS: Bone marrow mesenchymal stem cells from Sprague-Dawley rats were cultured and isolated in vitro, and interfered with different concentrations of VEGF (20, 40, 60, 80, 100, 120 μg/L) and PDGF-BB (20, 40, 60, 80, 100, 120 μg/L) in combination or 100 μg/L VEGF, and 100 μg/L PDGF-BB alone. The optimum concentration of promoting cell proliferation was detected by cell counting kit-8 assay. The expression levels of angiopoietin-1, hypoxia-inducible factor-1α, hepatocyte growth factor and insulin-like growth factor were detected by RT-PCR at 7 and 14 days after intervention.

RESULTS AND CONCLUSION: (1) After growth factors were added, the cell proliferative ability was significantly improved, and combined application revealed better effect. The optimal concentration grou was 80 μg/L VEGF+80 μg/L PDGF-BB. (2) Both VEGF and PDGF-BB could promote the mRNA expression levels of angiopoietin-1, hypoxia-inducible factor-1α, hepatocyte growth factor and insulin-like growth factor, and the effect was more obvious in combined application. (3) The mRNA expression levels of hypoxia-inducible factor-1α and hepatocyte growth factor were significantly increased with time (P < 0.05). The mRNA expression levels of angiopoietin-1 and insulin-like growth factor were significantly decreased with time (P < 0.05). (4) In vitro experimental results suggest that VEGF and PDGF-BB at the concentration of  80 μg/L can consistently promote angiogenesis, and the effect of combined application is better than that of single application.

Key words: bone marrow mesenchymal stem cells, vascular endothelial growth factor, platelet-derived growth factor-BB, tissue-engineered bone, angiogenesis

CLC Number: