Expression and significance of prolyl hydroxylase and von-Hippel-Lindau tumor suppressor protein in the synovial tissues of rheumatoid arthritis

doi:10.3969/j.issn.2095-4344.1305

Abstract

Abstract:

BACKGROUND: It is widely recognized that hypoxia-inducible factor plays a role in rheumatoid arthritis. As the key factors of hypoxia-inducible factors, whether prolyl hydroxylase and von-Hippel-Lindau tumor suppressor protein (pVHL) participate in rheumatoid arthritis still remains unknown.
OBJECTIVE: To investigate the relationship between prolyl hydroxylase and pVHL in the synovial tissues of rheumatoid arthritis and osteoarthritis, and to explore the underling mechanism.
METHODS: Synovial tissues were removed from 11 patients with rheumatoid arthritis undergoing knee arthroplasty, and synovial tissues from 20 patients with osteoarthritis undergoing knee arthroplasty were as controls. The study was approved by the Ethics Committee of Affiliated Hospital of Qinghai University, and the patients signed the informed consents. The expression levels of prolyl hydroxylases 1, 2 and 3, and pVHL in the synovial tissues were detected by immunohistochemical EnVision two-step method.
RESULTS AND CONCLUSION: (1) All of the prolyl hydroxylases 1, 2 and 3, and pVHL were positive in the two groups. Prolyl hydroxylase 1 was significantly lower in rheumatoid arthritis patients than in osteoarthritis patients (P < 0.01). The expression level of prolyl hydroxylase 2 in rheumatoid arthritis patients was significantly higher than those in synovial tissues of osteoarthritis (P < 0.05). There was no significant difference in the expression of hydroxylase 3 and pVHL between two groups (P > 0.05). (2) The analysis of Spearman rank correlation showed that hydroxylases 2 and 3 (rs=-0.875, P < 0.01), hydroxylase 1 and red blood cell distribution width-standard deviation (rs=-0.374, P < 0.03), hydroxylase 2 and hemoglobin (rs=-0.457, P < 0.01), hydroxylase 3 and red blood cell distribution width-standard deviation (rs=-0.363, P < 0.04) were negatively correlated respectively, while there were positive correlation between hydroxylase 2 and eosinophil (rs=0.363, P < 0.04), hydroxylase 2 and hemoglobin (rs=0.405, P < 0.02), respectively. (3) Rheumatoid factor, C-reactive protein, serum amyloid A, and globulin were signi?cantly higher in serum of rheumatoid arthritis patients than in those of the osteoarthritis patients (P < 0.05). (4) These results indicate that the changes of hydroxylases 1 and 2 in synovial tissues of rheumatoid arthritis patients might be involved in the pathogenesis of this disease, which can help us understand a way of molecular biology for the occurrence of rheumatoid arthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: rheumatoid arthritis, osteoarthritis, synovial tissues, prolyl hydroxylase, von-Hippel-Lindau tumor suppressor protein

CLC Number:

R446

Song Xiaoli1, Su Juan2, Liu Chongyang1, Zhang Guangyuan3, Zhang Xin4. Expression and significance of prolyl hydroxylase and von-Hippel-Lindau tumor suppressor protein in the synovial tissues of rheumatoid arthritis[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(23): 3623-3629.

Figures/Tables 5

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