Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (34): 6321-6325.doi: 10.3969/j.issn.2095-4344.2012.34.011

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In vitro cytotoxicity of the self-made light-cured nano-hydroxyapatite composite for tooth restoration evaluated by MTT assay

Li Rui1, Wang Qing-shan1, Wang Yun2, Zhao Meng-meng1, Wang Shuang1, Zhang Rong-he1   

  1. 1Department of Stomatology, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China;
    2Department of Stomatology, People’s Hospital of Liaocheng City, Liaocheng 252000, Shandong Province, China
  • Received:2012-02-04 Revised:2012-03-27 Online:2012-08-19 Published:2012-08-19
  • Contact: Wang Qing-shan, Professor, Master’s supervisor, Department of Stomatology, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China wqsyahao@ yahoo.com.cn
  • About author:Li Rui★, Master, Physician, Department of Stomatology, Affiliated Hospital, Binzhou Medical University, Binzhou 256603, Shandong Province, China Lirui19860724@ 163.com

Abstract:

BACKGROUND: More and more new materials are introduced in the field of oral application, and the evaluation of biomaterial biocompatibility is a key step before the clinical application
OBJECTIVE: To evaluate the biocompatibility of the self-made light-cured nano-hydroxyapatite composite for tooth restoration through in vitro cytotoxic experiments.
METHODS: According to ISO standards, in vitro cytotoxicity test (MTT assay) was used to test the absorbance value of human gingival fibroblasts in the self-made light-cured nano-hydroxyapatite composite for tooth restoration extracts (experimental group) of 1, 3, 5 days, to observe changes in cell morphology, to calculate the relative growth rate, and to determine the cytotoxicity grade. Cells in negative control group were cultured in Dulbecco’s modified Eagle’s medium, while those in positive control group were cultured in Dulbecco’s modified Eagle’s medium containing 0.1% phenol.
RESULTS AND CONCLUSION: The absorbance value in the experimental group was similar to that in the negative control group (P > 0.05), but significantly different from that in the positive control (P < 0.01), which indicated that the materials tested were safe to the human gingival fibroblasts. It is preliminarily estimated that nano-hydroxyapatite composite is a safe material for dental clinical application with good biocompatibility.

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