Artificial bone construction using bone marrow mesenchymal stem cells combined with calcium polyphosphate fiber reinforced calcium phosphate cement

doi:10.3969/j.issn.2095-4344.1502

Abstract

Abstract:

BACKGROUND: Calcium phosphate cement is a research focus of biomedical materials and has been widely used in clinical practice. However, the low compressive strength and bending strength of calcium phosphate cement limit its applications, and as the artificial bone, the lack of osteoinductive activity is also a major defect. Therefore, the enhancement of calcium phosphate cement has become a hot spot in this field.
OBJECTIVE: To explore the feasibility of constructing artificial bone by bone marrow mesenchymal stem cells combined with calcium phosphate fiber/bone cement.
METHODS: Calcium phosphate cement/calcium polyphosphate fiber composite scaffold materials at a reasonable proportion were prepared, in which calcium polyphosphate fibers accounted for 10%, 20%, 30%, 40%, 50% and 60% of the total mass respectively. Then, compression test, three-point bending test and torsion test were performed. The maximum compressive load and bending load were recorded, so as to determine the proportion of calcium phosphate/calcium polyphosphate fibers under the best strength. Bone marrow mesenchymal stem cells were isolated and cultured by density gradient centrifugation and induced by osteogenesis. The cultured cells were inoculated onto the calcium polyphosphate fiber/calcium phosphate cement composite scaffold at the most suitable proportion. The growth of bone marrow mesenchymal stem cells on the composite scaffold was observed by scanning electron microscope.
RESULTS AND CONCLUSION: The hardness and toughness of the composite scaffold material reached the best when the calcium phosphate fibers accounted for 20% of the total mass of the scaffold. Bone marrow mesenchymal stem cells grew well on the composite scaffold under the scanning electron microscope. Approximately 7 days after culture, the bone marrow mesenchymal stem cells were completely filled in the space of the composite scaffold. To conclude, the calcium polyphosphate fiber plays the role of “steel bar” in the composite scaffold, enhancing the strength and toughness of the scaffold material. The addition of bone marrow mesenchymal stem cells changes the osteogenesis from a single bone conduction mode to an osteogenic mode with bone conductivity, bone induction and bone generation, which enhances the biocompatibility of the scaffold material.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Calcium Phosphates, Bone Marrow, Mesenchymal Stem Cells, Tissue Engineering

CLC Number:

Cui Xintao, Yang Xiansheng, Chi Zhiyong, Guan Guofa. Artificial bone construction using bone marrow mesenchymal stem cells combined with calcium polyphosphate fiber reinforced calcium phosphate cement[J]. Chinese Journal of Tissue Engineering Research, 2019, 23(1): 74-78.

Figures/Tables 2

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