Biological characteristics of human placental mesenchymal stem cells isolated using two culture methods: a comparative study

doi:10.3969/j.issn.2095-4344.0644

Abstract

Abstract:

BACKGROUND: Placental mesenchymal stem cells can provide ideal seed cells for cell therapy, but trypsin cold digestion has not been used for cell culture. Therefore, we explored this method and compared it with the tissue explant method to screen a more convenient and more successful culture method.
OBJECTIVE: To observe the biological features of human placental mesenchymal stem cells isolated by trypsin cold digestion and tissue explant method.
METHODS: We isolated, purified, and subcultured mesenchymal stem cells (n=6) from human placenta by using trypsin cold digestion method and tissue explant method. The first appearance time and primary culture period of placental mesenchymal stem cells isolated using two methods were recorded. The growth curves of passage 3 cells from two culture methods were generated. Flow cytometry was used to analyze the expression of surface markers of passage 3 cells isolated using the two culture methods, and to study the neural and adipogenic differentiation of the cells.
RESULTS AND CONCLUSION: Placental mesenchymal stem cells could be obtained using both culture methods. Adherent cells in the trypsin cold digestion group appeared significantly earlier than those in the tissue explant group (P < 0.05), and the primary culture period was also significantly shorter in the trypsin cold digestion method (P < 0.05). The growth curve of passage 3 cells showed a higher cell number at plateau growth using trypsin cold digestion than tissue explant method (P < 0.05). The cells isolated using two culture methods were positive for CD29 and CD105, but negative for CD34 and CD45, indicating the same cell phenotypes. After the induction, placental mesenchymal stem cells cultured by both methods had the potential to differentiate into nerve cells and adipocytes. Therefore, both culture methods can produce placental mesenchymal stem cells, but trypsin cold digestion method is more suitable for the culture of this kind of cells as compared with the tissue explant method.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Placenta, Mesenchymal Stem Cells, Cell Culture Techniques, Tissue Engineering

CLC Number:

R394.2

Zhang Fei, Wang Wu, Li Hui-juan, Liu Ya-fei, Zhang Bao-gang, Pei Fu-qiang, Shao Qing-wei, Wu Zhong-yan. Biological characteristics of human placental mesenchymal stem cells isolated using two culture methods: a comparative study[J]. Chinese Journal of Tissue Engineering Research, 2018, 22(33): 5292-5296.

Figures/Tables 3

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