Umbilical cord mesenchymal stem cell transplantation increases microvessel density in surrounding areas of acute traumatic brain injury in rats

doi:10.3969/j.issn.2095-4344.2017.33.012

Abstract

Abstract:

BACKGROUND: Umbilical cord mesenchymal stem cells (UC-MSCs) are a kind of pluripotent stem cells capable of relieving inflammation and edema in injured areas, promoting microvascular regeneration and improving wound healing.
OBJECTIVE: To explore the therapeutic effects of UC-MSCs transplantation on acute traumatic brain injury (TBI) in rats and the effect on microvessel density of the rat brain tissue.
METHODS: Thirty Sprague Dawley rats were randomly divided into three groups, control group, TBI model group and UC-MSCs treatment group (n=10 per group). TBI models were made in the rats by free fall method. US-MSCs (1.5×10⁶) were infused through the cerebral ventricle in the rats. Neurological severity scores were assessed at 24 hours after cell transplantation. The microvessel density in the injured brain tissue was detected using immunohistochemistry method at 14 days after cell transplantation.
RESULTS AND CONCLUSION: Compared with the TBI model group, the microvessel density value was significantly increased after UC-MSCs transplantation (P < 0.05), and the neurological severity score also became better in the UC-MSCs treatment group (P < 0.05). In summary, UC-MSCs transplantation can effectively improve became vascular remodeling and have a good neuroprotection in acute TBI rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Craniocerebral Trauma, Umbilical Cord, Mesenchymal Stem Cell Transplantation, Tissue Engineering

CLC Number:

R394.2

Xu Hai-huan, Dong Hua-jiang, Shang Chong-zhi, Zhao Ming-liang. Umbilical cord mesenchymal stem cell transplantation increases microvessel density in surrounding areas of acute traumatic brain injury in rats[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(33): 5320-5324.

Figures/Tables 1

References

[1] 江基尧,朱波,罗其中.颅脑创伤临床救治指南[M]. 3版. 上海:上海第二军医大学出版社,2007:1-241.

[2] Schouten JW, Fulp CT, Royo NC, et al. A review and rationale for the use of cellular transplantation as a therapeutic strategy for traumatic brain injury. J Neurotrauma. 2004;21(11): 1501-1538.

[3] 陈镭,苗宗宁,张东强,等.人脐血源性间充质干细胞的体外培养及生物学鉴定[J].江苏医药,2005,31(7):481-483.

[4] Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143-147.

[5] Mareschi K, Biasin E, Piacibello W, et al. Isolation of human mesenchymal stem cells: bone marrow versus umbilical cord blood. Haematologica. 2001;86(10):1099-1100.

[6] Li JP, Wang DW, Song QH. Transplantation of erythropoietin gene-transfected umbilical cord mesenchymal stem cells as a treatment for limb ischemia in rats. Genet Mol Res. 2015; 14(4):19005-19015.

[7] 陈镭,惠国桢,张赛,等.人脐血间充质干细胞移植促进大鼠颅脑创伤后神经功能的恢复[J].中华创伤杂志,2009,25(6):498-502.

[8] Abboud A, Mi Q, Puccio A, et al. Inflammation Following Traumatic Brain Injury in Humans: Insights from Data-Driven and Mechanistic Models into Survival and Death. Front Pharmacol. 2016;7:342.

[9] Aerts JM, Haddad WM, An G, et al. From data patterns to mechanistic models in acute critical illness. J Crit Care. 2014;29(4):604-610.

[10] Centers for Disease Control and Prevention (CDC). CDC grand rounds: reducing severe traumatic brain injury in the United States. MMWR Morb Mortal Wkly Rep. 2013;62(27): 549-552.

[11] Newell E, Shellington DK, Simon DW, et al. Cerebrospinal Fluid Markers of Macrophage and Lymphocyte Activation After Traumatic Brain Injury in Children. Pediatr Crit Care Med. 2015;16(6):549-557.

[12] Hinson HE, Rowell S, Schreiber M. Clinical evidence of inflammation driving secondary brain injury: a systematic review. J Trauma Acute Care Surg. 2015;78(1):184-191.

[13] Medzhitov R. Origin and physiological roles of inflammation. Nature. 2008;454(7203):428-435.

[14] Maas AI, Stocchetti N, Bullock R. Moderate and severe traumatic brain injury in adults. Lancet Neurol. 2008;7(8): 728-741.

[15] McIntosh TK. Neurochemical sequelae of traumatic brain injury: therapeutic implications. Cerebrovasc Brain Metab Rev. 1994;6(2):109-162.

[16] Caplan AI.Mesenchymal stem cells. J Orthop Res. 1991;9(5): 641-650.

[17] Lozano D, Gonzales-Portillo GS, Acosta S, et al. Neuroinflammatory responses to traumatic brain injury: etiology, clinical consequences, and therapeutic opportunities. Neuropsychiatr Dis Treat. 2015;11:97-106.

[18] 迟作华,张洹,何冬梅,等.脐血间充质干细胞培养条件的优化[J].中国实用内科杂志:临床版,2006,26(5):372-375.

[19] Cui B, Li E, Yang B, et al. Human umbilical cord blood-derived mesenchymal stem cell transplantation for the treatment of spinal cord injury. Exp Ther Med. 2014;7(5):1233-1236.

[20] Tepper OM, Galiano RD, Kalka C, et al. Endothelial progenitor cells: the promise of vascular stem cells for plastic surgery. Plast Reconstr Surg. 2003;111(2):846-854.

[21] Pittenger MF, Mackay AM, Beck SC, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143-147.

[22] Freitas AL, Oliveira e Silva Md, Matsumoto PM, et al. Experimental model of obtaining tissue adipose, mesenchymal stem cells isolation and distribution in surgery flaps in rats. Acta Cir Bras. 2014;29 Suppl 2:29-33.

[23] 谷涌泉,韩忠朝,付小兵.干细胞临床研究与应用[M].北京:人民卫生出版社,2012.

[24] 韩忠朝.间充质干细胞基础与临床[M].北京:科学出版社,2012: 1-90.

[25] 陶凯,全亮亮.再生医学丛书-组织工程学[M].沈阳:辽宁科学技术出版社,2016:19-31.

[26] 刘新峰.脑血管病介入治疗学[M]. 2版.北京:人民卫生出版社, 2012:1-50.