Effects of different growth factors on the antioxidant capacity of endplate chondrocytes

doi:10.3969/j.issn.2095-4344.2017.04.005

Abstract

Abstract:

Abstract
BACKGROUND: It is well-known that vitamin E holds antioxidant capacity, but whether other growth factors have the same effect on endplate chondrocytes has not yet been reported.
OBJECTIVE: To observe the effect of different growth factors on the antioxidant ability of endplate chondrocytes in the intervertebral disc.
METHODS: Endplate chondrocytes were primary cultured, and then divided into four groups, including blank control, serum deprivation, hydrogen peroxide stimulation and hydrogen peroxide stimulation combined with different growth factors groups. The 4th group was subdivided into insulin-like growth factor-1, basic fibroblast growth factor, transforming growth factor β, forskolin and vitamin E groups. The expression levels of caspase-3, matrix metalloproteinase 13 and 3, inhibitor of metalloproteinase 1 as well as thrombin 4 and 5 were detected by real-time PCR. Cell apoptosis was analyzed through apoptosis kit and flow cytometry. Cell synthesis and secretion were detected by western blot assay. The total antioxidant capacity and the hydrogen peroxide content were determined by kit, and then statistically analyzed.
RESULTS AND CONCLUSION: Different growth factors had significant differences in the endplate chondrocyte apoptosis, secretion and antioxidant capacity. To conclude, transforming growth factor β and forskolin do further damage to the cells stimulated by hydrogen peroxide, while insulin-like growth factor-1 and vitamin E expose protective effect on the injured cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Intervertebral Disc, Insulin-Like Growth Factor I, Transforming Growth Factor beta, Vitamin E, Tissue Engineering

CLC Number:

R318

Huang Xiao-dong1, Deng Guo-ying2, Wang Wei-heng1, Xu Li-zhang1, Ma Jun1, Ye Xiao-jian1. Effects of different growth factors on the antioxidant capacity of endplate chondrocytes[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(4): 520-526.

Figures/Tables 4

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