Isolation and culture of primary microvascular endothelial cells from mouse brains

doi:10.3969/j.issn.2095-4344.2016.51.010

Abstract

Abstract:

BACKGROUND: Brain microvascular endothelial cells (BMECs) are important tools in the field of neuroscience research; therefore, how to obtain highly purified BMECs is a key and difficulty in vitro.
OBJECTIVE: To develop a simple method of isolating and culturing highly purified BMECs.
METHODS: C57BL/6 mice aged 6-8 weeks old were selected, and microvessels were obtained using enzyme digestion and gradient centrifugation. Further, endothelia cells were purified by certain drugs, followed by identified by CD31 and GFAP immunofluorescence staining. The expression of Claudin-5 was detected using immunofluorescence staining with anti-Claudin-5 antibody.
RESULTS AND CONCLUSION: Mouse BMECs grew and arranged in spiral or cobblestone-like. Immunofluorescence staining showed that the purity of BMECs reached above 99% and Claudin-5 was highly expressed. In conclusion, a simple method of easy accessibility is developed to obtain highly purified primary mouse BMECs.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Endothelial Cells, Cells, Cultured, Fluoroimunoassay, Tissue Engineering

CLC Number:

R318

Zhang Zuo-hui, Chen Chen, Chen Hao, Cui Gui-yun, Hua Fang. Isolation and culture of primary microvascular endothelial cells from mouse brains[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(51): 7666-7671.

Figures/Tables 2

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