Biological characteristics of bone marrow mesenchymal stem cells in patients with or whithout coronary heart disease: a comparative study

doi:10.3969/j.issn.2095-4344.2016.50.005

Abstract

Abstract:

BACKGROUND: It is unclear whether the body differences in patients with different types of heart diseases affect the characteristics and performance of stem cells.
OBJECTIVE: To explore the biological characteristics of bone marrow mesenchymal stem cells from patients with different types of heart diseases.
METHODS: Bone marrow mesenchymal stem cells were extracted using density gradient centrifugation from the bone marrow of 27 patients with coronary heart diseases and 20 patients with other heart diseases. Cell morphology was observed in the two groups. CD13, CD34, CD45, CD54, CD106 and CD44 positive expression was detected by flow cytometry. Cell proliferation was detected by MTT method, and the in vitro cell growth curves of the two groups were described.
RESULTS AND CONCLUSION: The bone marrow mesenchymal stem cells of the two groups showed a long spindle shape, and there was no significant difference in the cell morphology between the two groups. In all the patients, the positive rates of CD34 and CD455 were less than 3.0%, while the positive rates of CD13 and CD44 were higher than 95.0%. However, the positive rates of CD54 and CD106 were higher in patients with coronary heart disease as compared with those with other heart diseases (P < 0.05). The in vitro growth curves of cells in the two groups were basically consistent, and the cell proliferation was only a little higher in the patients with other heart diseases compared with those with coronary heart disease. Experimental results show that different types of heart diseases in patients have no influence on morphology and proliferation of bone marrow mesenchymal stem cells, but some function-related proteins may exhibit certain difference in their expressions.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Coronary Disease, Tissue Engineering

CLC Number:

R394.2

Hou Yong-lan. Biological characteristics of bone marrow mesenchymal stem cells in patients with or whithout coronary heart disease: a comparative study[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(50): 7481-7486.

Figures/Tables 3

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