Biological properties of bone morphogenetic proteins and basic fibroblast growth factor in biological materials for repair of articular cartilage defect

doi:10.3969/j.issn.2095-4344.2016.20.004

Abstract

Abstract:

BACKGROUND: Articular cartilage regeneration can be regulated by autocrine or paracrine secretion of various cytokines.
OBJECTIVE: To analyze biological properties of bone morphogenetic proteins and basic fibroblast growth factor in biological materials for repair of articular cartilage defect.
METHODS: Forty New Zealand white rabbits were used and equally randomized into four groups: fibrin, basic fibroblast growth factor, bone morphogenetic protein, and combined treatment (basic fibroblast growth factor combined with bone morphogenetic protein) groups, respectively. Bioactive scaffolds with fibrin, basic fibroblast growth factor, bone morphogenetic protein, and basic fibroblast growth factor combined with bone morphogenetic protein were injected to repair the articular cartilage defect. Therapeutic effect and biological properties of biological materials were compared.
RESULTS AND CONCLUSION: (1) In the fibrin group, two rabbits appeared to have limps. In the basic fibroblast growth factor group hand function was limited in one rabbit. In the bone morphogenetic protein group, one had a limp and one was in a limitation of activity. In the combined treatment group, rabbits recovered well and showed no differences in the knee joint before and after surgery (P < 0.05). (2) General observation: In the combined treatment group, soft solution cartilage defects disappeared, and angiogenesis and cartilage were similar with normal tissues. In the bone morphogenetic protein group, fractured cartilage marginal existed and could not be closely integrated with normal cartilage. The presence of chondrocytes in the periphery of the defect was seen under light microscope. In the fibrin group, defect site and surrounding tissues healed. In the basic fibroblast growth factor group, defect was repaired, but not smooth. (3) Results of hematoxylin and eosin staining: In the fibrin group, the bone defect was not repaired, obvious depression surface was seen. In basic fibroblast growth factor group, repair of cartilage defect was obvious. There were a lot of chondrocytes. In the bone morphogenetic protein group, the bone defect was repaired; chondrocytes appeared, but irregular arrangement. In the combined treatment group, good bone defect repair and a large number of cartilage cells were seen. Taken together, biological materials with fibrin and basic fibroblast growth factor are ideal for repair of articular cartilage defect by promoting formation of cartilage by bone morphogenetic protein and enhancing chondrocyte proliferation by basic fibroblast growth factor.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0002-0170-1787(董君博)

Key words: Joints, Cartilage, Bone Morphogenetic Proteins, Fibroblast Growth Fator2

CLC Number:

R318

Dong Jun-bo1, 2. Biological properties of bone morphogenetic proteins and basic fibroblast growth factor in biological materials for repair of articular cartilage defect[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(20): 2915-2920.

Figures/Tables 2

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