Simvastatin effects on the expressions of specific osteogenic genes in bone marrow stromal stem cells

doi:10.3969/j.issn.2095-4344.2016.19.006

Abstract

Abstract:

BACKGROUND: Previous studies have demonstrated that simvastatin that can promote osteogenic differentiation of bone marrow stromal stem cells in vitro, is likely to be a new osteogenic drug. While it is still unknown whether there is time-dependent stimulation of simvastatin on the expressions of bone morphogenetic protein 2 and collagen type I.
OBJECTIVE: To investigate the expressions of bone morphogenetic protein 2 and collagen type I in rat bone marrow stromal stem cells in vitro stimulated by simvastatin at different time points.
METHODS: Passage 1 bone marrow stromal cells were divided into control and simvastatin group, followed by cultured in osteogenic differetiation medium with or uithout 10-7 mol/L simvastatin. After 7-day intervention, expression of alkaline phosphatase was detected in passage 3 cells. Passage 4 cells were divided and cultured as described above, and afterwards, RNA and proteins were extracted at 12 and 36 hours to detect the expressions of bone morphogenetic protein 2 and collagen type I using real-time PCR and western blot assay.
RESULTS AND CONCLUSION: Both two groups could express alkaline phosphatase, while the rate of positive cells significantly increased in the simvastatin group compared with the control group (P < 0.05); at 12 and 36 hours after intervention, mRNA expressions of bone morphogenetic protein 2 and collagen type I in the simvastatin group were significantly higher than those in the control group (P < 0.05). Besides, western blot assay showed: at both 12 and 36 hours, simvastatin significantly enhanced the expression of bone morphometric protein 2, while the expression of collagen type I significantly increased at 12 hours (P < 0.05), but not at 36 hours. In conclusion, simvastatin can promote the expressions of bone morphometric protein 2 and collagen type I in rat bone marrow stromal cells, with more favorable outcomes after 12-hour treatment.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Simvastatin, Bone Morphogenetic Proteins, Collagen Type I, Tissue Engineering

CLC Number:

R394.2

Liu Hao, Zhang Yan, Liu Jia-yin, Liu Guang-yuan, Zhang Ke-zhong, Zhang Guo-bin, Xing Lei,Tian Fa-ming. Simvastatin effects on the expressions of specific osteogenic genes in bone marrow stromal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(19): 2777-2782.

Figures/Tables 1

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