Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (1): 78-82.doi: 10.3969/j.issn.2095-4344.2016.01.014

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Feasibility of umbilical cord blood stem cell transplantation for the treatment of diabetic lower limb ischemia

Xie Li-hua1, Xing Lin2, Zheng Hang3   

  1. 1Affiliated Central Hospital of Xinxiang Medical University, Xinxiang 453000, Henan Province, China; 2Cell Research Center, Zhengzhou University Medical School, Zhengzhou 450001, Henan Province, China; 3Third Affiliated Hospital of Xinxiang Medical University, Xinxiang 453003, Henan Province, China
  • Received:2015-11-29 Online:2016-01-01 Published:2016-01-01
  • Contact: Xing Lin, Professor, Cell Research Center, Zhengzhou University Medical School, Zhengzhou 450001, Henan Province, China
  • About author:Xie Li-hua, Attending physician, Affiliated Central Hospital of Xinxiang Medical University, Xinxiang 453000, Henan Province, China

Abstract:

BACKGROUND: Diabetic lower limb ischemia is prone to involve distal lower limb arteries, and a conventional treatment is often unable to obtain the ideal effect.
OBJECTIVE: To investigate the effect and safety of umbilical cord blood stem cell transplantation in the treatment of diabetic lower limb ischemia.
METHODS: A diabetic rat model of lower limb ischemia was established, and along the femoral artery, five points 
were selected for injection of human umbilical cord mesenchymal stem cell suspension, 20 μL per point. At 1, 2, 4 weeks after transplantation, transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region, and incidence of adverse reactions were recorded.
RESULTS AND CONCLUSION: At 1, 2 and 4 weeks after transplantation, the transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region were found increasing, which were significantly different from those before transplantation (P < 0.05). At different time after transplantation, all animals had no inflammatory reactions such as skin bleeding and dermatitis, and local red, swelling, hot, pain, and had no tumor-like growth in organs. These findings indicate that umbilical cord blood stem cell transplantation can safely and significantly improve symptoms of diabetic lower limb ischemia, which has certain application feasibility.
 

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