Therapeutic effect of Fukeyangkun pills and bone marrow mesenchymal stem cells transplantation on thin endometrium

doi:10.3969/j.issn.2095-4344.2016.01.012

Abstract

Abstract:

BACKGROUND: Studies have found that endometrial cells from bone marrow donors can be detected in the endometrium of female patients after bone marrow suppression.

OBJECTIVE: To investigate the effect of Fukeyangkun pills combined with bone marrow mesenchymal stem cells (BMSCs) transplantation in the treatment of thin endometrium.

METHODS: Thirty female rats at rutting period were randomized into control, model, Fukeyangkun pills, cell transplantation and combined group (n=6 per group). Rat models of thin endometrium were made in the latter five groups. Rats in the six groups were respectively subjected to routine feeding, tail vein injection of normal saline, tail vein injection of bone marrow mesenchymal stem cells (1 mL) at 6 hours and 10 days after modeling, intragastric administration of 5 mL/kg Fukeyangkun pill solution for continuous 20 days, or tail vein injection of bone marrow mesenchymal stem cells (1 mL) at 6 hours and 10 days after modeling plus intragastric administration of 10 mL/kg Fukeyangkun pill solution for continuous 20 days. At 21 days after modeling, hematoxylin-eosin staining was used to observe the morphological changes of the endometrical tissues and measure the endometrium thickness. The expression of cytokeratin and vimentin was determined by western blot assay.

RESULTS AND CONCLUSION: Compared with the model group, the endometrium thickness and the expression of cytokeratin and vimentin (P < 0.05) were increased successively in the Fukeyangkun pills group, cell transplantation group, and combined group to different extents. Of these groups, the endometrium thickness and the expression of cytokeratin and vimentin in the combined group were the most close to normal levels. Our data demonstrate that bone marrow mesenchymal stem cell transplantation can induce regeneration of the endometrial cells and repair endometrial tissue. Furthermore, treatment of Fukeyangkun pills obviously augments the repair effect of bone marrow mesenchymal stem cell transplantation on thin endometrium.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Han Ran, Dai Ning, Lin Yun, Lin Tong. Therapeutic effect of Fukeyangkun pills and bone marrow mesenchymal stem cells transplantation on thin endometrium[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(1): 65-69.

Figures/Tables 4

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