Comparing three methods of preparing tissue engineering tracheal matrix

doi:10.3969/j.issn.2095-4344.2013.02.001

Abstract

Abstract:

BACKGROUND: It is a key step to prepare tracheal matrices in tissue engineering for repairing long segment tracheal defects.
OBJECTIVE: To find the better method for preparing tissue engineering tracheal matrices through comparing and analyzing three different ways of preparing allogeneic acellular tracheal matrices.
METHODS: Fresh rabbit tracheas were obtained by surgical operation and divided to four groups: untreated group, conventional vitrificational group, detergent-enzymatic group, modified vitrificational group. Hematoxylin-eosin staining and scanning electron microscopy were used to observe the morphology and ultrastructure of the treated tracheal matrices. The biomechanical properties including maximum tensile force, rupture force and tissue elongation of the treated tracheal matrices were measured.
RESULTS AND CONCLUSION: Histological observations showed that there were epithelial cells in the untreated and conventional vitrificational groups, but there were no epithelial cells in the detergent-enzymatic and modified vitrificational groups. There were abundant extracellular matrices and collagen fibers in the untreated group, conventional vitrificational group, and modified vitrificational group under electromicroscopy. Conversely, no extracellular matrix was found in the detergent-enzymatic group, in which there were only collagen fibers. Finally, no statistical differences were found in the maximum tensile force, rupture force and tissue elongation among all the groups. The method of modified vitrification, by which the antigen can be removed, and the extracellular matrix and biomechanical properties can be maintained effectively, is a more ideal way to prepare tissue engineering tracheal matrix.

Key words: tissue construction, basic experiments of tissue construction, tracheal scaffold, enzymatic washing, conventional vitrificational method, modified vitrificational method, extracellular matrix, tissue construction photographs-containing paper

CLC Number:

R318

Wu Fan, Zhang Zhi-pei, Wang Jian, Li Jian-zhong, Jin Yan, Jiang Tao, Han Yong, Li Xiao-fei. Comparing three methods of preparing tissue engineering tracheal matrix[J]. Chinese Journal of Tissue Engineering Research, 2013, 17(2): 191-195.

Figures/Tables 6

References

[1] Teng Z, Trabelsi O, Ochoa I, et al. Anisotropic material behaviours of soft tissues in human trachea: An experiment alstudy. J Biomech. 2012; 45(9):1717-1723.

[2] Badylak SF, Weiss DJ, Caplan A, et al. Engineered whole organs and complex tissues. J Lancet. 2012;379(9819): 943-952.

[3] Baiguera S, Del Gaudio C, Jaus MO, et al. Long-term changes to in vitro preserved bioengineered human trachea and their implications for decellularized tissues. J Biomaterials. 2012;33(14):3662- 3672.

[4] Yang B, Zhou L, Peng B, et al. Stem cells in a tissue-engineered human airway. J Lancet. 2012; 379(9825): 1487.

[5] Jungebluth P, Bader A, Baiguera S,et al. The concept of in vivo airway tissue engineering. J Biomaterials. 2012;33(17): 4319-4326.

[6] Liang ZB, Li XF, Liu K. Zhongguo Zuzhi Gongcheng Yanjiu yu Linchuang Kangfu. 200;12(5):929-932.梁志波,李小飞,刘锟.同种异体气管移植的研究及展望[J].中国组织工程研究与临床康复,2008,12(5):929-932.

[7] Xiaofei Li, Jian Wang, Yunfeng Ni, et al. Bone morphogenetic protein-2 stimulation of cartilage regeneration in canine tracheal graft. J Journal of Lung and Heart Transplantation. 2009;28(3):285- 289.

[8] Hinderer S, Schesny M, Bayrak A, et al. Engineering of fibrillar decorin matrices for a tissue-engineered trachea. J Biomaterials. 2012;33(21):5259-5266.

[9] Daly AB, Wallis JM, Borg ZD, et al. Initial binding and recellularization of decellularized mouse lung scaffolds with bone marrow-derived mesenchymal stromal cells. J Tissue Eng Part A. 2012; 18(2):1-16.

[10] Shi HC, Xu H, Wu J, et al. Zhonghua Waike Zazhi. 2008; 46(20):1589-1590.史宏灿,徐洪,吴俊,等. 玻璃化冷冻同种异体气管移植动物模型的建立[J].中华外科杂志,2008,46(20):1589-1590.

[11] Go T, Jungebluth P, Baiguero S, et al. Both epithelial cells and mesenchymal stem cell-derived chondrocytes contribute to the survival of tissue-engineered airway transplants in pigs. J Thorac Cardiovasc Surg. 2010;139(2):437-443.

[12] Zhang WH, Huo R, Li SB, et al. Zhongguo Meirong Yixue. 2010;7(19):1015-1017.张文浩,霍然,李尚滨, 等.组织工程化犬气管的生物力学研究[J].中国美容医学,2010,7(19):1015-1017.

[13] Macchiarini P, Baiguera S , Jungebluth P, et al. Tissue engineered human tracheas for in vivo implantation. J Biomaterials. 2010;31(34):8931-8938.

[14] Macchiarini P, Jungebluth P, Go T, et al. Clinical transplantation of a tissue- engineered airway. J Lancet. 2008; 372(9655):2023-2030.

[15] Xu H, Shi HC, Zang WF, et al. An experimental research on cryopreserving rabbit trachea by vitrification. J Cryobiology. 2009;58(2):225-231.

[16] Yavin S, Arav A. Measurement of essential physical properties of vitrification solutions. J Theriogenology.2007; 67(1):81-89.

[17] Guan J, Urban JP, Li ZH, et al．Effects of rapid cooling on articular cartilage. J Cryobiology. 2006;52(3):430-439.

[18] Dahl SL, Chen Z, Solan AK, et al. Feasibility of vitrification as a storage method for tissue-engineered blood vessels. J Tissue Eng.2006;12(2):291-300.