Salidroside promotes osteogenic differentiation of MC3T3-E1 cells: an in vitro experiment

doi:10.12307/2025.207

Abstract

Abstract: BACKGROUND: Bone defects can directly affect the success rate and long-term stability of dental implants. Studies have shown that salidroside has the ability to promote the proliferation and differentiation of osteoblasts, but less is reported on its pathways related to osteogenic differentiation.
OBJECTIVE: To investigate the effects of salidroside on the proliferation and differentiation of MC3T3-E1 cells and the expression of related genes and proteins through in vitro cell experiments.
METHODS: Cell counting kit-8 test and alkaline phosphatase test were used to determine the optimal concentration of salidroside (0.5, 1, 5, 10, and 50 μmol/L) in promoting the proliferation and differentiation of MC3T3-E1 cells. There were four groups in the experiment: control group, salidroside group, salidroside+ LY294002 group, and LY294002 group, which were cultured with osteogenic induction solution, osteogenic induction solution containing 10 μmol/L salidroside, osteogenic induction solution containing 10 μmol/L salidroside+10 μmol/L LY294002, and osteogenic induction solution containing 10 μmol/L LY294002, respectively. The effects of salidroside and LY294002, an inhibitor of the PI3K/Akt signaling pathway, on the expressions of genes and proteins related to osteogenesis were observed.
RESULTS AND CONCLUSION: Cell counting kit-8 assay and alkaline phosphatase assay showed that salidroside promoted the proliferation of MC3T3-E1 cells most significantly at 10 μmol/L. Compared with the control group, salidroside could promote mineralization, promote cell adhesion, reduce cell death, increase mRNA expression of Runx-2, osteocalcin and osteopontin (P < 0.01), and increase protein expression of Runx-2 and p-Akt (P < 0.01). However, the addition of LY294002 reversed the above results. These findings indicate that salidroside can promote the mineralization of MC3T3-E1 cells and the expression of osteogenesis-related genes and proteins, which may be related to the activation of PI3K/Akt signaling pathway.

Key words: salidroside, MC3T3-E1 cell, cell differentiation, osteogenesis, mineralization, LY294002, PI3K/Akt signaling pathway

CLC Number:

Liu Zhaohui, Han Xiaoqian, Duan Xin, Guo Pengda, Zhang Yuntao. Salidroside promotes osteogenic differentiation of MC3T3-E1 cells: an in vitro experiment[J]. Chinese Journal of Tissue Engineering Research, 2025, 29(2): 231-237.

Figures/Tables 10

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