Chinese Journal of Tissue Engineering Research ›› 2023, Vol. 27 ›› Issue (18): 2831-2836.doi: 10.12307/2023.317
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Liu Xiaoyun1, Deng Yuping1, 2, 3, Li Feifei1, Zhao Dongliang3, 4, Yang Yang1, Huang Tao1, 5, Tan Wenchang3, 4, Wu Yaobin1, Huang Wenhua1, 6, Li Yanbing1
Received:
2022-05-05
Accepted:
2022-06-06
Online:
2023-06-28
Published:
2022-09-17
Contact:
Li Yanbing, MD, Professor, Master’s supervisor, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China
About author:
Liu Xiaoyun, Master candidate, Guangdong Engineering Research Center for Translation of Medical 3D Printing Application, Guangdong Provincial Key Laboratory of Medical Biomechanics, National Key Discipline of Human Anatomy, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, Guangdong Province, China
Supported by:
CLC Number:
Liu Xiaoyun, Deng Yuping, Li Feifei, Zhao Dongliang, Yang Yang, Huang Tao, Tan Wenchang, Wu Yaobin, Huang Wenhua, Li Yanbing. Effect of elastin degradation of patellar tendon on the quasi-static tensile mechanical properties[J]. Chinese Journal of Tissue Engineering Research, 2023, 27(18): 2831-2836.
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2.4 组织学染色观察结果 组织学染色分析弹性蛋白酶对髌腱中弹性蛋白纤维和胶原蛋白纤维的影响。VVG染色将弹性蛋白纤维染成深黑色,将胶原纤维染成红色,与PBS处理组相比,弹性蛋白酶处理的组织清楚显示弹性蛋白降解效果,见图3A,B。Masson染色将胶原蛋白纤维染成蓝色,显示弹性蛋白酶处理前后的胶原纤维方向不受酶处理的影响,显示了弹性蛋白沿着胶原纤维分布,胶原纤维呈波浪状,卷曲清晰可见,见图3C,D。在弹性蛋白酶处理之前,组织中的胶原纤维和弹性纤维排列整齐紧凑;弹性蛋白酶处理后,组织变得疏松,弹性蛋白在组织中零碎存在,胶原纤维波浪状的卷曲度减少。除此之外,还可见弹性蛋白酶从外表面开始向内降解弹性蛋白。"
2.5.2 两组髌腱处理前后应力松弛 图5显示了PBS对照组和弹性蛋白酶组处理前后的峰值应力、平衡应力、松弛百分比。PBS对照组处理后的峰值应力和平衡应力比处理前的小,峰值应力降低约37%,平衡应力降低约43%,差异有显著性意义(P=0.001,P < 0.000 1),应力下降幅度差异无显著性意义(P=0.241),见图5A。弹性蛋白酶组处理后的峰值应力和平衡应力比处理前的小,峰值应力降低约41%,平衡应力降低约61%,差异有显著性意义(P < 0.000 1),应力下降幅度增加约12%,差异有显著性意义(P=0.007),见图5B。两组应力随时间的变化趋势显示,与处理前相比,组织经过处理后表现出明显下降趋势,应力值差异有显著性意义(P值均< 0.000 1),见图6A,B。通过对相应组内所有样品的归一化应力取平均值绘制平均归一化应力-时间曲线,见图6C,显示了弹性蛋白酶组比PBS对照组引起的应力降低值更大(P < 0.000 1)。"
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