Chinese Journal of Tissue Engineering Research ›› 2022, Vol. 26 ›› Issue (35): 5658-5664.doi: 10.12307/2022.886
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Luo Shiren, Xie Yan, Zhang Li, Yin Na
Received:
2021-10-11
Accepted:
2021-11-29
Online:
2022-12-18
Published:
2022-05-16
Contact:
Xie Yan, Associate researcher, Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang 471002, Henan Province, China
About author:
Luo Shiren, Master, Pharmacist-in-charge, Henan Provincial Luoyang Orthopedic-Traumatological Hospital (Henan Provincial Orthopedic Hospital), Luoyang 471002, Henan Province, China
Supported by:
CLC Number:
Luo Shiren, Xie Yan, Zhang Li, Yin Na. miRNA screening for targeted regulation of bone growth by semen ziziphi spinosae extract[J]. Chinese Journal of Tissue Engineering Research, 2022, 26(35): 5658-5664.
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2.6 生物信息学网站预测与芯片筛选的调控5-HT2AR的优选miRNA 由于给药后小鼠体长增长时5-HT2AR低表达,所以观察影响5-HT2AR表达并在酸枣仁提取物组中上调的miRNAs。结果miRNADB网站预测mmu-miR-34a-5p可以调控5-HT2AR表达,与芯片法筛选结果相同。作者用RNAhybrid网站筛查结果显示mmu-miR-34a-5p与5-HT2AR有很好的结合性。 2.7 qRT-PCR法验证mmu-miR-34a-5p在酸枣仁提取物组和对照组之间的差异表达 RNA的纯度结果显示,A260 nm/A280 nm比值介于1.8-2.0之间,说明提取的RNA合格。qRT-PCR结果显示酸枣仁提取物组mmu-miR-34a-5p的表达水平较空白对照组明显升高(P < 0.05),见表6。"
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