Chinese Journal of Tissue Engineering Research ›› 2021, Vol. 25 ›› Issue (25): 3956-3961.doi: 10.12307/2021.004

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Effects of human growth hormone on proliferation and osteogenic differentiation of human periodontal ligament stem cells

Yang Junhui1, 2, Luo Jinli1, 2, Yuan Xiaoping1, 2   

  1. 1Department of Orthodontics of Stomatological Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; 2Laboratory of Oral and Maxillofacial Reconstruction and Regeneration of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2020-09-03 Revised:2020-09-05 Accepted:2020-10-22 Online:2021-09-08 Published:2021-03-24
  • Contact: Yuan Xiaoping, Professor, Department of Orthodontics of Stomatological Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Laboratory of Oral and Maxillofacial Reconstruction and Regeneration of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:ang Junhui, Master candidate, Physician, Department of Orthodontics of Stomatological Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China; Laboratory of Oral and Maxillofacial Reconstruction and Regeneration of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:
    the Luzhou City Government-Sichuan Medical University Joint Project, No. 2015LZCYD-S05(1/12) (to YXP)

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Abstract: BACKGROUND: Growth hormone has been proven to promote the proliferation and bone differentiation of non-dental mesenchymal stem cells, but the biological effect on human periodontal ligament stem cells is still unclear.
OBJECTIVE: To explore the effect of human growth hormone on the proliferation and osteogenic differentiation of human periodontal ligament stem cells. 
METHODS: The α-MEM complete medium containing 0 (control group), 10, 100, 200 μg/L human growth hormone was added to the third generation of human periodontal ligament stem cells. CCK-8 assay was used to detect cell proliferation at 1, 3, 5, and 7 days. The osteoinductive fluids containing 0 (control group), 10, 100, 200 μg/L human growth hormone was added to the third generation of human periodontal ligament stem cells. At 7 days, real-time PCR and western blot assay were used to detect the gene and protein expression of the bone differentiation-related factors opn and runx2. At 14 days, Alizarin Red staining and Alizarin Red semi-quantitative detection were used to analyze the mineralization of human periodontal ligament stem cells in the late stage of osteogenic formation.    
RESULTS AND CONCLUSION: The cell proliferation of 100 and 200 μg/L groups was significantly better than that of the control group (P < 0.05). The gene and protein expression of opn and runx2 was higher in the 100 and 200 μg/L groups than that in the control group (P < 0.05). The mineralization of human periodontal ligament stem cells in the 100 and 200 μg/L groups was better than that of the control group (P < 0.05). Results confirm that in vitro experiments, human growth hormone at concentrations of 100 and 200 μg/L can promote the proliferation and osteogenic differentiation of human periodontal ligament stem cells. 

Key words: stem cells, human periodontal ligament stem cells, human growth hormone, proliferation, differentiation, osteogenesis, factor

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