Effects of 20-minute warm ischemia on coronary endothelial function of pig donor hearts after cardiac death

doi:10.3969/j.issn.2095-4344.2016.29.015

Abstract

Abstract:

BACKGROUND: Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation.
OBJECTIVE: To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD).
METHODS: Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left anterior descending coronary artery or combined with heart preservation pretreatment for 4 hours was performed in the DCD and DCD plus cold storage groups. The maximum coronary endothelium-dependent relaxation was determined in the three groups.
RESULTS AND CONCLUSION: There were no significant differences in the maximum coronary endothelium-dependent relaxation and the minus logarithmic of substance concentration induced 50%maximal relaxation among three groups (P > 0.05). These results indicate that 20-minute warm ischemia cannot lead to obvious coronary endothelial dysfunction. In addition, DCD combined with 4-hour cold storage does not affect coronary endothelial function.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Heart, Transplantation, Endothelial Cells, Swine

CLC Number:

R318

Sun Xiao-ke, Zhang Wei-hua, Qin Guang-qi, Liao Qiu-ming, Stig Steen, Qiao Chen-hui. Effects of 20-minute warm ischemia on coronary endothelial function of pig donor hearts after cardiac death[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(29): 4362-4367.

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