Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (29): 4362-4367.doi: 10.3969/j.issn.2095-4344.2016.29.015

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Effects of 20-minute warm ischemia on coronary endothelial function of pig donor hearts after cardiac death

Sun Xiao-ke1, Zhang Wei-hua1, Qin Guang-qi2, Liao Qiu-ming2, Stig Steen2, Qiao Chen-hui1   

  1. 1Department of Cardiac Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; 2Department of Cardiothoracic Surgery, Lund University Hospital, Sweden 22594
  • Received:2016-04-21 Online:2016-07-08 Published:2016-07-08
  • Contact: Qiao Chen-hui, M.D., Professor, Chief physician, Department of Cardiac Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
  • About author:Sun Xiao-ke, Attending physician, Department of Cardiac Surgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China
  • Supported by:

    the Key Science and Technology Program of Henan Province, China, No. 142102310346

Abstract:

BACKGROUND: Myocardial and coronary endothelial injury occurs in donor hearts due to warm ischemia during cardiac transplantation. Coronary endothelial structure and function play a critical role in long-term outcomes for patients after cardiac transplantation. 
OBJECTIVE: To study the effect of hypoxia-induced warm ischemia (20 minutes) on coronary endothelial function of porcine donor hearts after cardiac death (DCD).
METHODS: Sixteen healthy Swedish domestic pigs were randomized into control (n=6), DCD (n=5), and DCD plus cold storage (n=5) groups, respectively. A DCD model in pigs was established using the method of hypoxia-induced 20-minute warm ischemia in the DCD and DCD plus cold storage groups. Isolation of the heart left anterior descending coronary artery or combined with heart preservation pretreatment for 4 hours was performed in the DCD and DCD plus cold storage groups. The maximum coronary endothelium-dependent relaxation was determined in the three groups.
RESULTS AND CONCLUSION: There were no significant differences in the maximum coronary endothelium-dependent relaxation and the minus logarithmic of substance concentration induced 50%maximal relaxation among three groups (P > 0.05). These results indicate that 20-minute warm ischemia cannot lead to obvious coronary endothelial dysfunction. In addition, DCD combined with 4-hour cold storage does not affect coronary endothelial function.

中国组织工程研究杂志出版内容重点:组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松组织工程

Key words: Heart, Transplantation, Endothelial Cells, Swine

CLC Number: