Effects of bone morphogenetic protein-2 combined with dexamethasone on proliferation and osteogenic differentiation of human gingival fibroblasts

doi:10.3969/j.issn.2095-4344.2014.51.010

Abstract

Abstract:

BACKGROUND: It is novel research field in repairing periodontal tissue defects by human gingival fibroblasts combined with cytokines.

OBJECTIVE: To investigate the effect of bone morphogenetic protein-2 combined with dexamethasone on the proliferation and osteogenic differentiation of human gingival fibroblasts.

METHODS: Human gingival fibroblasts at passage 3 were divided into five groups: DMEM, basal osteogenic medium, basal osteogenic medium and dexamethasone, basal osteogenic medium and bone morphogenetic protein-2, basal osteogenic medium, dexamethasone and bone morphogenetic protein-2 groups. Cell proliferation was detected by MTT. Osteogenic differentiation was examined by alkaline phosphatase activity, alizarin red staining and reverse transcription PCR.

RESULTS AND CONCLUSION: Human gingival fibroblasts had the potency of osteogenic differentiation. Dexamethasone and bone morphogenetic protein-2 had no significant influence on the proliferation of human gingival fibroblasts. Alkaline phosphatase activity and calcium deposition were promoted by dexamethasone or bone morphogenetic protein-2, especially by both together. Combination of dexamethasone and bone morphogenetic protein-2 can promote the expression of osteogenic related-genes, which enhances the osteogenic differentiation of human gingival fibroblasts effectively.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

全文链接：

Key words: tooth, bone morphogenetic proteins, dexamethasone, fibroblasts, cell proliferation

CLC Number:

R318

Tao Yu-fei, Jiang Shao-yun, Yan Zhi-min, Zhen Zhen, Deng Jia-yin. Effects of bone morphogenetic protein-2 combined with dexamethasone on proliferation and osteogenic differentiation of human gingival fibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(51): 8248-8253.

Figures/Tables 5

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