Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (8): 1359-1362.doi: 10.3969/j.issn.1673-8225.2012.08.007

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Rapid detection of Schistosoma Japonicum by using polydiacetylene nanoparticles

Zhou Wei-hua1, 2, Guo Xun-zhi1, 2, Zhang Yang-de1, He Jian-tai1   

  1. 1National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha  410008, Hunan Province, China; 2Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun  336000, Jiangxi Province, China
  • Received:2011-12-08 Revised:2011-12-20 Online:2012-02-19 Published:2012-02-19
  • About author:Zhou Wei-hua☆, Doctor, Associate professor, National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha 410008, Hunan Province, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China subdeb@163.com Guo Xun-zhi, Studying for master’s degree, Lecturer, National Hepatobiliary & Enteric Surgery Research Center, Ministry of Health, Institute of Biomedical Engineering of Central South University, Changsha 410008, Hunan Province, China; Key Laboratory of Jiangxi Province for Research on Active Ingredients in Natural Medicines, Yichun University, Yichun 336000, Jiangxi Province, China
  • Supported by:

     the Science Supporting Program of Jiangxi Province, No. 2009BSA12400*; the Science and Technology Project of Jiangxi Education Bureau, No. GJJ09359*

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Abstract:

BACKGROUND: PCR, ELISA, immunofluorescence-ball method and gene chip technology for rapid detection of Schistosoma Japonicum will need hours, volume requirements of Schistosoma Japonicum, specialists and certain equipments to get results, and are not suitable for on-site rapid test.
OBJECTIVE: To prepare polydiacetylene nanoparticles for detection of Schistosoma Japonicum.
METHODS: Polydiacetylene nanoparticles (PDA) were prepared using sonication.
RESULTS AND CONCLUSION: The prepared nanoparticles had uniform particle size, narrow distribution and stable structure. Dilution of rabbit serum after schistosome infection was dropped into PDA nanoparticle dilution, and the solution color turned from blue to red. Twenty seconds later, 70%-80% of the solution changed from blue to red. The whole solution changed in 2 minutes. The graphs of absorption spectroscopy, transmission electron microscopy, and nanosizer displayed big changes with Schistosoma Japonicum and antibody PDA nanoparticle. It is indicated that colorimetric detection of Schistosoma Japonicum using PDA nanoparticles is simple and rapid.

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