Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (8): 1363-1366.doi: 10.3969/j.issn.1673-8225.2012.08.008

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Endocytosis and exocytosis of gold nanochain attaching Hep-2 cells of human laryngeal carcinoma*☆

Cong Lin-hai1, He Xiao-guang1, Yang Yi-bing1, Zhang Shi-wen2, Peng Shu-kun1   

  1. 1Department of Otorhinolaryngology, First Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China; 2Department of Head and Neck Surgery, Third Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China
  • Received:2011-07-16 Revised:2011-08-26 Online:2012-02-19 Published:2012-02-19
  • Contact: He Xiao-guang, Professor, Department of Otorhinolaryngology, First Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China hexg1018@ yahoo.com.cn
  • About author:Cong Lin-hai☆, Doctor, Attending physician, Department of Otorhinolaryngology, First Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China conglinhai2008@ 126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81160324/H1625*

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Abstract:

BACKGROUND: The gold nanoparticles have a killing effect on tumor cells.
OBJECTIVE: To investigate the influence of gold nanochain on Hep-2 cells proliferation of human laryngeal carcinoma.
METHODS: The gold nanochain was prepared by a glucose synthesis method and added into the culture cells with different concentrations (10%, 25%, 50%, 75%, 95%) to test the influence on proliferation of in vitro cultured Hep-2 cells. The endocytosis and exocytosis of transmembrane when gold nanochain attached to Hep-2 cells were observed by electron microscopy.
RESULTS AND CONCLUSION: The gold chain at high concentrations (75%, 95%) exhibited inhibitory effects on the proliferation of Hep-2 cells, but the influence was not increased with increasing concentration, belonging to a range of non-toxic. Gold nanchain can enter into Hep-2 cells after 8 hours of co-culture and leave cells after 48 hours, indicating gold nanoparticles chain can enter and leave Hep-2 freely.

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