Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 998-1002.doi: 10.3969/j.issn.1673-8225.2012.06.011

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Role of stromal cell derived factor-1 in proliferation and migration of endogenous neural stem cells after traumatic brain injury

Wang Chong-qian, Ding Peng, Mu Lin-jie, Shang Ya-jun, Li Hui, Wang Jin-kun   

  1. Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming  650032, Yunnan Province, China
  • Received:2011-07-08 Revised:2011-09-01 Online:2012-02-05 Published:2012-02-05
  • Contact: Wang Jin-kun, Chief physician, Master’s supervisor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • About author:Wang Chong-qian, Associate chief physician, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China 19wolf@163.com Ding Peng☆, Doctor, Associate professor, Department of Neurosurgery, the First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China Mulinjie84@gmail.com
  • Supported by:

    General Project of Applied Basic Research of Yunnan in 2008, No. 2008ZC132M*

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Abstract:

BACKGROUND: The migration of neural stem cells plays an important role in brain injury, but its specific mechanism is still unclear.
OBJECTIVE: To observe the role of stromal cell derived factor-1 (SDF-1) in proliferation and migration of endogenous neural stem cells after traumatic brain injury in rats.
METHODS: The left motor and sensory cortical areas in rats were hit by self-made free-fall drop using Feeney’s method in order to establish the moderate and severe traumatic brain injury model. The brains were removed at 6 hours, 3, 5 and 7 days after injury. Immunohistochemical analysis of Nestin and SDF-1 was performed on frozen sections. The correlation between proliferation and migration of endogenous neural stem cells and SDF-1 was analyzed. Control group and sham-operated group were taken as control.
RESULTS AND CONCLUSION: The difference of neuroethology assessments between the brain injury group and the control group was significant. Immunohistochemical analysis on frozen sections showed: the SDF-1 was increased significantly compared with the control group at 3 days after injury, a great amount of Nestin-positive cells were seen in the hippocampus and none in the injured region. The SDF-1 expression was increased and distributed more obviously and widely at 5 and 7 days, and few Nestin-positive cells were seen in the injured region. There were no changes in the control and sham-operated groups. It is indicated that the expression of SDF-1 is increased as time going on after brain injury, and SDF-1 is involved in the proliferation and migration of endogenous neural stem cells to the injured region.

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