Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 994-997.doi: 10.3969/j.issn.1673-8225.2012.06.010

Previous Articles     Next Articles

Bromodeoxyuridine assay for detecting the proliferation of neural progenitor cells in mice at different ages

Zhu Qian1, Zhao Yun-he1, Liu Xue-qin1, Yang Wu-lin2, Lu Li1   

  1. 1Department of Anatomy, Shanxi Medical University, Taiyuan  030001, Shanxi Province, China; 2School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei  230009, Anhui Province, China
  • Received:2011-08-27 Revised:2011-10-19 Online:2012-02-05 Published:2012-02-05
  • Contact: Lu Li, Doctor, Associate professor, Master’s supervisor, Department of Anatomy, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China luli7300@126.com
  • About author:Zhu Qian★, Studying for master’s degree, Department of Anatomy, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China zhuqian-20404@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30973094*; Youth Science and Technology Foundation of Shanxi Province, No. 2009021045*; Innovation Foundation of Shanxi Medical University, No. 201007*; the National Natural Science Foundation of China, No. 30800178*

PDF

453

Cited

1

Abstract:

BACKGROUND: Bromodeoxyuridine (BrdU) is an analog of thymidine that can be used to analyze the proliferation of neural progenitor cells (NPCs) in the forebrain subventricular zone (SVZ).
OBJECTIVE: To systematically assess the proliferation of NPCs in SVZ of mice at different ages by using the optimal BrdU labeling method.
METHODS: Embryonic (n=5), neonatal (n=5), adult (n=5) and aged mice (n=5) were used in this study. Mice were given intraperitoneal injection of BrdU, the proliferation of NPCs in forebrain cells in rats at different ages was analyzed, and the influence of niche on NPCs proliferation was investigated.
RESULTS AND CONCLUSION: The number of BrdU+ cells in SVZ of embryonic, neonatal, adult and aged mice was (897.20±22.38), (262.17±26.26), (76.67±5.63) and (43.8±2.61), respectively. The proliferation of NPCs was decreased as the age increased (P < 0.05). Meanwhile, SA-β-galactosidase staining was used to detect senescence cells in the forebrain，positive cells were found around ventricle in adult and aged mice. Increased activity of SA-β-gal in the brain indicated that age-related changes may influence the proliferation of NPCs.

CLC Number: