Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (6): 1007-1010.doi: 10.3969/j.issn.1673-8225.2012.06.013

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Biological characteristics and significance of CD133+ cancer stem cells in human nasopharyngeal carcinoma 

Wang Hai-li, Wen Zhong, Sheng Cong-xiang, Zhong Pin-neng, Wang Jun-qi   

  1. Department of Otolaryngology Head and Neck Surgery, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou  510282, Guangdong Province, China
  • Received:2011-11-10 Revised:2011-12-22 Online:2012-02-05 Published:2012-02-05
  • Contact: Wen Zhong, Doctor, Professor, Department of Otolaryngology Head and Neck Surgery, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China wenzhong60 @163.com
  • About author:Wang Hai-li★, Studying for master’s degree, Department of Otolaryngology Head and Neck Surgery, Affiliated Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China wanghaili.12345@163.com
  • Supported by:

    the Natural Science Foundation of Guangdong Province, No. S2011010003947*; Science and Technology Program Project of Guangdong Province, No. 2008B030301344*

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Abstract:

BACKGROUND: Recent studies shows that cancer stem cells exist in cancer cell lines, and may be responsible for recurrence and metastasis of tumor. There are few reports on the biological characteristics of CD133+ cancer stem cells in human nasopharyngeal carcinoma.
OBJECTIVE: To explore the biological characteristics and significance of CD133+ cancer stem cells in human nasopharyngeal carcinoma.
METHODS: Expression of the CD133+ cells were detected by flow cytometry, and purified by magnetic cell sorting from human nasopharyngeal carcinoma cell line CNE-2. In vitro proliferation and in vivo tumorigenic capacity of the CD133+ stem cells were detected by serum-free culture, CCK-8, plate colony formation test and tumorigenicity experiment in nude mice, and which statistically compared with CD133- and control CNE-2 cells to understand the biological characteristics of the CD133+ stem cells.
RESULTS AND CONCLUSION: CD133+ cells maintained a sphere-like growth status in serum-free medium in vitro, and could form tumor spheres. Compared with the CD133- cells, the CD133+ cells had higher colony-formation ability (P < 0.01). In vivo tumorigenesis ability of the CD133+ cells in the nude mice was higher than that of the CD133- cells (P = 0.05). The results confirmed that CD133+ cells can successfully separate and culture in vitro, and can generate tumor spheres and have high tumorigenesis ability in the nude mice.

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