Abstract:

BACKGROUND: The researchers have recognized that there is a very close relationship between the activation of the Toll-like receptors and organ transplant rejection, and the Toll-like receptors and transplantation immunology have gradually become a hot topic.
OBJECTIVE: To observe the changes of Toll-like receptors in the serum drawing from rabbits treated with recombinant human interleukin-10 after allogeneic skin grafting.
METHODS: The rabbit back defect models were prepared, and the receptor rabbits were divided into six groups: low dose recombinant human interleukin-10 group, high dose recombinant human interleukin-10 group, low dose cyclosporine A group, high dose cyclosporine A group, joint group and normal saline group. The 5 and 10 μg/(kg•d) recombinant human interleukin-10, 5 and 10 mg/(kg•d) cyclosporine A, 5 μg recombinant human interleukin-10+5 mg cyclosporine A and 1 mL/d normal saline were injected into the rabbits respectively from 1 day before grafting and lasted for 10 days.
RESULTS AND CONCLUSION: The levels of serums Toll-like receptor 4, Toll-like receptor 7, Toll-like receptor 8 and Toll-like receptor 9 were increased after skin grafting compared with that before grafting and reached to the peak concentration on 14 days. At 4 and 7 days after grafting, the level of Toll-like receptors 4 in the normal saline group was higher than that in the joint group (P < 0.05). At 7 days after grafting, the level of Toll-like receptor 7 in the normal saline group was higher than that in the other five groups (P < 0.05), and the level of Toll-like receptor 8 in the normal saline group was higher than that in the high dose cyclosporine A group (P < 0.05). There was no significant difference of Toll-like receptor 9 level in each two groups at different time points after grafting (P > 0.05). The levels of serum Toll-like receptor 4, Toll-like receptor 7, Toll-like receptor 8 and Toll-like receptor 9 were increased obviously after the rabbits skin grafting which have treated with recombinant human interleukin-10 resisting skin grafting, and reached to the peak concentration after skin graft rejection, and then decreased gradually, which means Toll-like receptors participate in the allograft rejection.