Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (5): 761-765.doi: 10.3969/j.issn.1673-8225.2012.05.001

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Establishing a rat model of orthotopic liver transplantation 

Shi Jun, Wu Qin-rong, Luo Wen-feng, Wang Yong-gang   

  1. Department of Organ Transplant, Jiangxi People’s Hospital, Nanchang  330006, Jiangxi Province, China
  • Received:2011-07-23 Revised:2011-10-20 Online:2012-01-29 Published:2012-01-29
  • About author:Shi Jun☆, Doctor, Professor, Master’s supervisor, Department of Organ Transplant, Jiangxi People’s Hospital, Nanchang 33 0006, Jiangxi Province, China shijun6207@163.com
  • Supported by:

     the Major Discipline Academic and Technology Leader Develop Project of Jiangxi Province, No. 2008DD01100*

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Abstract:

BACKGROUND: Establishing a model of orthotopic liver transplantation by two-cuff technique can decrease the time without the liver and significantly increase the survival rate of the rats subjected to liver transplantation.
OBJECTIVE: To establish the rats models of orthotopic liver transplantation based on two-cuff technique in combination with some related literatures.
METHODS: Totally 150 Sprague Dawley rats‎ were treated with liver transplantation by improved two-cuff method in the portal vein, hepatic inferior vena cava under the law in line with cuff, liver superior and inferior vena cava were sutured with suture line. Biliary tract reconstruction was completed in bile duct stent law.
RESULTS AND CONCLUSION: Receptors rats were generally in good condition after operation. 1-day survival rate was 94.0% and 1-week survival rate was 90.0% in 50 cases of the formal experiment. The time for donor operation and receptor operation was (34.44±3.25) minutes and (49.07±4.93) minutes, respectively, and anhepatic time was (17.26±2.51) minutes. The average blocking time of inferior vena cava was 20 minutes. To master the operation technique, operate patiently and carefully and reduce various complications can get a stable rat model of orthotopic liver transplantation.
 

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