Abstract:

BACKGROUND: Bacteria adhere on the prosthesis surface and form a biological membrane, so the drugs cannot kill the bacteria. It is the main reason for refractory treatment and recurrence of artificial joint replacement infections.
OBJECTIVE: To investigate the influence of properties and surfaces of different artificial joint prosthesis materials on the adhesion abilities of Mycobacterium tuberculosis.
METHODS: Specimens of titanium alloy and cobalt chromium molybdenum alloy were made respectively (with smooth surface and rough surface), Mycobacterium tuberculosis was labeled by FITC after subculture in order to make into bacterial liquid; at the same time, the Staphylococcus epidermidis was collected for control study. Bacteria liquid and samples were co-cultured at 37 ℃ for 24 hours and then observed using fluorescence microscope and scanning electron microscope to identify the adhesion conditions of these two bacteria.
RESULTS AND CONCLUSION: The adhesion areas of Staphylococcus epidermidis and Mycobacterium tuberculosis on the cobalt chromium molybdenum alloy were larger than that on the titanium alloy; in the case of the same material, the rough surface was more prone to bacterial adhesion than the smooth surface; there was biofilm formation for Staphylococcus epidermidis on the surface of two kinds of materials, while there was no biofilm formation for Mycobacterium tuberculosis. This study suggests that the Mycobacterium tuberculosis has poor adhesion ability on the surface of cobalt chromium molybdenum alloy and titanium alloy; the property and the roughness of the material have tremendous effects on the adhesion abilities of Mycobacterium tuberculosis; and there is no biofilm formation for Mycobacterium tuberculosis on the surface of cobalt chromium molybdenum alloy and titanium alloy.