Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (47): 8794-8797.doi: 10.3969/j.issn.2095-4344.2012.47.012

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Effects of basic fibroblast growth factor nanospheres on the proliferation of bone marrow mesenchymal stem cells

Wang Zeng-rong1, Zhang Yong-hong1, Li Er-feng1, Lu Xing1, Zhao Liang-qi2   

  1. 1Department of Orthopedics, Second Clinical College of Shanxi Medical University, Taiyuan 030006, Shanxi Province, China
    2Institute of Biotechnology of Shanxi University, Taiyuan 030006, Shanxi Province, China
  • Received:2012-03-04 Revised:2012-05-03 Online:2012-11-18 Published:2013-03-15
  • Contact: Zhang Yong-hong, Master’s supervisor, Chief physician, Department of Orthopedics, Second Clinical College of Shanxi Medical University, Taiyuan 030006, Shanxi Province, China
  • About author:Wang Zeng-rong★, Studying for master’s degree, Physician, Department of Orthopedics, Second Clinical College of Shanxi Medical University, Taiyuan 030006, Shanxi Province, China 371174251@qq.com

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Abstract:

BACKGROUND: A new-type poly 3-hydroxybutyrate-co-3-hydroxyoctanoate (PHBHOx) has been developed by fermentation technology, which not only possesses general characteristics but also has impnoued flexibility and processing performance.
OBJECTIVE: To detect the effect of PHBHOx encapsulated basic fibroblast growth factor (bFGF) nanospheres on the proliferation of bone mesenchymal stem cells (BMSCs).
METHODS: PHBHOx encapsulated bFGF nanospheres were prepared by W1/O/W2 ultrasound emulsification method. Then, BMSCs were cultured by whole bone marrow method. After that the culture solution was divided into three groups: bFGF group, nanospheres group and control group according to its different ingredients. The effective concentration of bFGF in the former two groups was set to 10, 20 and 50 μg/L, respectively.
RESULTS AND CONCLUSION: At days 1 and 3, there was no significant difference in absorbance value between bFGF group and nanospheres group (P > 0.05), but their absorbance value was both higher than that of the control group (P < 0.05); that is bFGF had promotive effect on BMSCs proliferation. At days 5 and 7, the absorbance value of the nanospheres group was higher than that of the bFGF group (P < 0.01), that was nanospheres could slowly relieve bFGF and obviously improve bioavailability. At day 10, nanospheres group showed strong proliferation capacity, and there was significant difference between the nanospheres group and the other two groups (P < 0.01). Meanwhile, bFGF group and control group had no significant difference (P > 0.05). These results suggest that there is a good sustained-release effect of nanospheres on bFGF and can play durable biological effects to promote BMSCs proliferation continually.

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