Abstract:

BACKGROUND: It is the focus and hotspot for the research of tissue engineering scaffold materials to prepare substitutes for the extracellular matrix with cell recognition and bionic scaffolds.
OBJECTIVE: To prepare and filter a porous three-dimensional scaffold for osteochondral tissue engineering which can meet the requirement and to evaluate its biocompatibility.
METHODS: Four kinds of scaffolds: collagen-chitosan, gelatin-chondroitin-sulfuric acid-sodium hyaluronate, collagen-ceramic bone and gelatin-ceramic bone were made by different biomaterials and divided into 1, 2, 3 and 4 groups, respectively. The fresh articular cartilage served as the control group.
RESULTS AND CONCLUSION: In group 1, bore diameter was 50-200 μm, and porosity was (90.5±2.1)%. In group 2, bore diameter was 100-150 μm, and porosity was (90.5±2.1)%. In group 3, bore diameter was 400-500 μm, and porosity was (67.5±2.1)%. In group 4, bore diameter was 300-400 μm, and porosity was (65.9±1.2)%. Mechanical test showed that the group 2 and group 4 basically met the experimental requirement: the structure and biochemistry components in groups 2, 3 and 4 were similar to the natural extracellular matrix, and could imitate extracellular microenvironment. These findings suggest that the gelatin-chondroitin-sulfuric acid-sodium hyaluronate and gelatin- ceramic bone scaffolds can be used as the scaffolds for composite tissues.