Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (46): 8698-8702.doi: 10.3969/j.issn.2095-4344.2012.46.030

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Effects of chemical pretreatment on hypoxic preconditioned mice

Zhang Jian-jun1, Sui Xin2, Lü Guo-wei3, Zhang Yan-bo4, Shao Guo5   

  1. 1Department of Emergency, Kangbashi Hospital Ordos 017000, Inner Mongolia Autonomous Region, China
    2Department of Neurosurgery, Baogang Hospital, Baotou 014010, Inner Mongolia Autonomous Region, China
    3Institute for Hypoxia Medicine, Capital Medical University, Beijing 100069, China
    4Department of Neurology, Affiliated Hospital of Taishan Medical University, Taian 271000, Shandong Province, China
    5Biomedical Research Center, Baotou Medical College, Baotou 014060, Inner Mongolia Autonomous Region, China
  • Received:2012-01-09 Revised:2012-03-16 Online:2012-11-11 Published:2013-03-16
  • Contact: Shao Guo, Doctor, Professor, Biomedical Research Center, Baotou Medical College, Baotou 014060, Inner Mongolia Autonomous Region, China shao_guo_china@163.com Zhang Yan-bo, Master, Associate professor, Department of Neurology, Affiliated Hospital of Taishan Medical University, Taian 271000, Shandong Province, China bbnnbn@163.com
  • About author:Zhang Jian-jun★, Master, Associate chief physician, Department of Emergency, Kangbashi Hospital Ordos 017000, Inner Mongolia Autonomous Region, China Sui Xin★, Master, Attending physician, Department of Neurosurgery, Baogang Hospital, Baotou 014010, Inner Mongolia Autonomous Region, China Zhang Jian-jun and Sui Xin contributed equally to this study.

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Abstract:

BACKGROUND: Hypoxia-inducible factor-1 α (HIF-1α) regulates the expression of genes which increase resistance to hypoxia tolerance. CoCl2 is a chemical reagent that can stabilize HIF-1α.
OBJECTIVE: To detect the effects of CoCl2 pretreatment on acute repetitive hypoxic exposure of mice.
METHODS: Balb/c mice were randomly divided into chemical pretreatment group and normal group. At 3 hours before treatment, the mice in the two groups were respectively injected with CoCl2 and normal saline. After that, the mice in the two groups were subjected to hypoxic exposure for 0 run (normal control group), 1 run, and 4 runs, respectively. Subsequently, hippocampi of mice were removed immediately after the exposure for index detection.
RESULTS AND CONCLUSION: The tolerance time after one-time hypoxic exposure in the chemical pretreatment group was higher than that in the control group, but there was no significant difference in four times hypoxic exposure between the chemical pretreatment and control groups. HIF-1 DNA binding activity of the four times hypoxic exposure subgroup in the control group was significantly higher than that of the one-time hypoxic exposure subgroup in the chemical pretreatment group and the normal control group. There was significant difference in each group. HIF-1 DNA binding activity of the one-time hypoxic exposure subgroup in the chemical pretreatment group was obviously higher than that in the normal group (P < 0.01). In the control group, erythropoietin and vascular endothelial growth factor mRNA level in the one-time hypoxic exposure and four times hypoxic exposure subgroups showed increased. Each group had significant difference (P < 0.05). There was no significant difference in erythropoietin and vascular endothelial growth factor mRNA levels among the chemical pretreatment group. These results suggest that CoCl2 chemical pretreatment can improve the tolerance time in hypoxic exposure, and thereby reduce induction of tolerance after hypoxic preconditioning and HIF-1 DNA binding activity.

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