Abstract:

BACKGROUND: Streptolysin O (SLO)-induced cell permeability is reversible to a certain extent, and the pores can be closed in the medium containing Ca2+.
OBJECTIVE: To observe whether SLO could improve the permeability of pig fibroblasts and to observe the states of cells treated with SLO of different concentrations.
METHODS: Pig fibroblasts were permeated with SLO solution of different concentrations (0, 230, 300 and 400 μg/L respectively). After incubation of 50 minutes, the cells were stained with PI to observe cell permeability. When adding the medium containing Ca2+, the cells were plated. Morphology and multiplication capacity of these cells were detected.
RESULTS AND CONCLUSION: Along with increase of SLO solution concentration, the cell permeability improved slightly. When cells were treated with SLO, the cell morphology was not changed. 230 μg/L SLO had no influence on the capacity of cell proliferation, and however, 300 μg/L and 400 μg/L SLO reduced the proliferation capacity significantly appearing statistically difference when compared with 0 μg/L group. These findings indicate that 230 μg/L SLO could increase cell permeability with keeping the normal shape and proliferation ability of cells.