Abstract:

BACKGROUND: The progression of primary osteoarthritis is the result of abnormal expression of various genes. We use mRNA gene chip technique to detect the synovial gene expression of knee osteoarthritis, which provides evidence for related pathological mechanism of knee osteoarthritis.
OBJECTIVE: To compare the alteration of synovial gene expression in patients with knee osteoarthritis and normal people in Yanbian area, and to evaluate the potential osteoarthritis related discriminating genes and their roles in pathological mechanism.
METHODS: This study included nine patients with knee osteoarthritis and three healthy controls. mRNA gene chip technique was used to analyze the gene expression profile of synovial tissue in these subjects and GO analysis was performed.
RESULTS AND CONCLUSION: The results of gene expression in the synovial tissue of osteoarthritis patients and healthy controls group showed that 1 261 genes were differently expressed, including 451 up-regulated genes and 810 down-regulated genes. The GO analysis showed that the gene expression about signal transduction, regulation of transcription, chemotaxis, inflammatory response, and cell proliferation was different. Gene chip technique is effective for screening associated genes, and the pathological progression of osteoarthritis is a complex progression which many genes are involved.