Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (34): 6372-6376.doi: 10.3969/j.issn. 2095-4344.2012.34. 021

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Expression of brain-derived neurotrophic factor and nerve growth factor mRNA in the co-culture of schwann cells and dental implants

Sun Lan-ying1, Li Zhao-yuan2, Ge Wen-zhang1, Wang Qi-bao3, Wang Hao4   

  1. 1Department of Prosthodontics,
    2Department of Oral and Maxillofacial Surgery,
    3Department of Medicine, Stomatological Hospital of Jinan, Jinan 250001, Shandong Province, China;
    4Experimental Center of Basic Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China
  • Received:2012-01-04 Revised:2012-02-15 Online:2012-08-19 Published:2012-08-19
  • Contact: Li Zhao-yuan, Master, Chief physician, Department of Oral and Maxillofacial Surgery, Stomatological Hospital of Jinan, Jinan 250001, Shandong Province, China lizhaoyuan60@ 163.com
  • About author:Sun Lan-ying★, Master, Attending physician, Department of Prosthodontics, Stomatological Hospital of Jinan, Jinan 250001, Shandong Province, China sunxiup@163.com

Abstract:

BACKGROUND: Schwann cells are probably the effective factors which can promote peripheral nerve regeneration of osseointegrated dental implants.
OBJECTIVE: To investigate the expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor (NGF) mRNA in the co-culture of Schwann cells and dental implants, and to analyze the biocompatibility of dental implants and Schwann cells.
METHODS: Wallerian degenerated sciatic nerves were harvested from adult dogs. Schwann cells were cultured by enzymatic digestion and differential adhesion methods. 1×108/L cells culture was then seeded onto the dental implants with titanium sandblasted and acid-etched surface as well as petri dish. The expression of BDNF and NGF mRNA in Schwann cells was detected by reverse-transcription PCR at days 1, 3, 6, 8 and 11, respectively.
RESULTS AND CONCLUSION: BDNF and NGF mRNA had a similar expression pattern with one peak either in the co-cultured Schwann cells or Schwann cells cultured alone. However, compared with the Schwann cells cultured alone, the expression levels of BDNF and NGF mRNA were higher and earlier peak appeared in the co-cultured Schwann cells. These findings suggest that dental implants with titanium sandblasted and acid-etched surface can promote the gene expression of Schwann cells. There is an excellent biocompatibility between this kind of dental implants and Schwann cells.

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