Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (25): 4682-4685.doi: 10.3969/j.issn.1673-8225.2012.25.025

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Detection of basement membrane components on the surface of acellular porcine cornea

Lin Xu-chu1, Jin Yan2, Hui Yan-nian3   

  1. 1Department of Ophthalmology, the 303 Hospital of Chinese PLA, Nanning 530021, Guangxi Zhuang Autonomous Region, China;
    2Research Center of Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China;
    3Department of Ophthalmology, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
  • Received:2011-11-12 Revised:2011-12-01 Online:2012-06-17 Published:2013-11-04
  • About author:Lin Xu-chu☆, Doctor, Associate chief physician, Department of Ophthalmology, the 303 Hospital of Chinese PLA, Nanning 530021, Guangxi Zhuang Autonomous Region, China linxuc2006@126.com

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Abstract:

BACKGROUND: The previous experiments have suggested that the acellular porcine cornea stroma (APCS) has a good compatibility and can support the growth of keratocytes and skin epithelial cells.
OBJECTIVE: To detect whether the important structure for the growth of corneal epithelial cells-basement membrane can be preserved on the surface of the APCS.
METHODS: Fluorescent antibody was used to detect the basement membrane component (laminin and collagen Ⅳ) by immunohistochemistry. Fluorescence microscopy was observed whether the basement membrane could be preserved on the surface of the APCS.
RESULTS AND CONCLUSION: Immunofluorescence staining showed that laminin and collagen Ⅳ positively expressed on the surfaces of porcine corneal stroma with the same level of fresh normal porcine corneal stroma. The results indicate the APCS can preserve the natural basement membrane component of the cornea which is beneficial to the growth of corneal epithelial cells.

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