Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3551-3555.doi: 10.3969/j.issn.1673-8225.2012.19.027

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RNA interference inhibits caspase-3 gene expression in bone marrow mesenchymal stem cells   

Liu Li-bao1, Hua Ping1, Yang Song-ran2, Liu Jia-liang1, Jiang Hui-qi1, Wang Meng1, Zeng Kuan1, Ao Xiang1, Yang Yan-qi1   

  1. 1Department of Cardiothoracic Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China; 2Department of Neurology, Guangzhou First Municipal People’s Hospital, Guangzhou 510180, Guangdong Province, China
  • Received:2011-10-30 Revised:2012-01-28 Online:2012-05-06 Published:2012-05-06
  • Contact: Hua Ping, M.D., Associate professor, Associate chief physician, Master’s supervisor, Department of Cardiothoracic Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China huaping88@sina.com
  • About author:Liu Li-bao★, Studying for master’s degree, Department of Cardiothoracic Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, Guangdong Province, China
  • Supported by:

     the Natural Science Foundtion of Guangdong Province, No. 7001619*; the Social Development Science and Technology Program of Guangdong Province, No. 2007B050200011*

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Abstract:

BACKGROUND: Poor viability of bone mesenchymal stem cells after transplantation into the infarcted region has limited the reparative capacity of these cells because of the apoptosis.
OBJECTIVE: To construct eukaryotic vector expressing small interfering RNAs against caspase-3 and investigate the inhibitory effect on the expression of caspase-3 in BMSCs in vitro.
METHODS: According to the principle of siRNA design, siRNAs against caspase-3 gene were designed and synthesized. Eukaryotic vector expressing caspase-3siRNA was constructed and transferred into BMSCs with the liposome conduct. There were 5 groups, including normal cell group, negative sequence group and 3 interfering groups (si-caspase-3-1, si-caspase-3-2 and si-caspase-3-3).
RESULTS AND CONCLUSION: Eukaryotic vector expressing caspase-3siRNA was constructed and transferred into BMSCs successfully. Western blot analysis and reverse-transcription polymerase chain reaction demonstrated that levels of of caspase-3 protein and mRNA in transfected BMSCs decreased, with the most significant decline in the group with si-caspase-3-3. Eukaryotic vector expressing caspase-3 siRNA could inhibit the expression of caspase-3 gene in BMSCs in vitro.

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