Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (19): 3556-3559.doi: 10.3969/j.issn.1673-8225.2012.19.028

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Effects of ginsenoside Rg3 on proliferation and induced differentiation of erythroleukemia cell line K562 in vitro 

Xiao Feng1, 2, Liu Bin1, Zhang Jian-ping2, Wang Ban-qing2, Fang Mu-shui2, Hu Wei2, Sun Xu-Lu2, Zhu Qing-Xian1   

  1. 1Medical College of Nanchang University, Nanchang  330006, Jiangxi Province, China; 2Medical College of Jinggangshan University, Jian 343000, Jiangxi  Province, China
  • Received:2011-12-11 Revised:2012-03-05 Online:2012-05-06 Published:2012-05-06
  • Contact: Zhu Qing-xian, Professor, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China
  • About author:Xiao Feng★, Master, Professor, Medical College of Nanchang University, Nanchang 330006, Jiangxi Province, China xiaofeng1008@yahoo.com.cn
  • Supported by:

    the Natural Science Foundation of Jiangxi Province, No. 2009GZY0123*; Science and Technology Topic of Education Department of Jiangxi Province, No. GJJ09337*

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Abstract:

BACKGROUND: Evidence exists that ginsenoside Rg3 can inhibit the growth of carcinoma cells. However, there are fewer studies describing ginsenoside Rg3 effects on leukemia.
OBJECTIVE: To investigate the effects of ginsenoside Rg3 on proliferation of erythroleukemia cell line K562 and the underlying mechanism.
METHODS: Erythroleukemia cell line K562 was used as target cell. The cells were divided into a control group and an Rg3 group. 10, 20, 40, 80, 100 mg/L Rg3 was added in the Rg3 group.
RESULTS AND CONCLUSION: The MTT colorimetric assay indicated that K562 growth inhibition rate was significantly higher in the Rg3 groups than in the control group (P < 0.05-0.01). The nitroblue tetrazolium assay showed that after culture for 1, 2, 3 days, the reducing power of K562 cells in the Rg3 group was significantly higher than in the control group (P < 0.01). In the Rg3 group, some K563 cell somas became small, nucleoli disappeared, nitroblue tetrazolium positive cells increased, and cells developed toward maturation. Flow cytometry detection showed that after 2 days of culture, the cell cycle at G2 phase in Rg3 group was 3.84 times higher than that in the control group. Ginsenoside Rg3 can inhibit the proliferation of erythroleukemia cell line K562 in vitro. The underlying mechanism may be related to a fact that ginsenoside Rg3 blocks K562 cell cycle at G2 stage, hinders normal mitosis and thereby inhibits cell proliferation.

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