Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (16): 2909-2912.doi: 10.3969/j.issn.1673-8225.2012.16.014

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Preparation and in vitro release of polylactic acid nanoparticles loaded with isoniazid and rifampicin 

Ding Xiao-li1, Wang Zi-li1, Ge Zhao-hui1, Wang Wen-ping2, Ma Xiao-ming1, Niu Ning-kui1, Li Ping1, Yang Xiao-ying3   

  1. 1Department of Orthopedics, 3Department of Pharmacy, General Hospital of Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China; 2College of Pharmacy, Ningxia Medical University, Yinchuan  750004, Ningxia Hui Autonomous Region, China
  • Received:2011-10-13 Revised:2011-12-14 Online:2012-04-15 Published:2012-04-15
  • Contact: author: Ge Zhao-hui, Master, Associate professor, Associate chief physician, Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China myovid@126.com
  • About author:Ding Xiao-li★, Master, Physician, Department of Orthopedics, General Hospital of Ningxia Medical University, Yinchuan 750004, Ningxia Hui Autonomous Region, China ufo-dingxiaoli@ 163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30960391*; the Natural Science Foundation of Ningxia Hui Autonomous Region, No. NZ09127*

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Abstract:

BACKGROUND: Micro-carrier drugs receive much concern based on their targeting, controlled-release, stability and good safety.
OBJECTIVE: To study release characteristics of polylactic acid nanoparticles loaded with isoniazid and rifampicin (INH-RFP-PLA-NPs) in vitro.
METHODS: INH-RFP-PLA-NPs were prepared with pusing modified spontaneous emulsification solvent diffusion method. The submicron particle size analyzer was used to detect the nanoparticle size and its distribution of INH-RFP-PLA-NPs. Observation of the morphology of INH-RFP-PLA-NPs was performed with transmission electron microscope. High performance liquid chromatography was used to detect drug loading and encapsulation efficiency. Phosphate buffer solution was used as a release medium to study the in vitro release characteristics of INH-RFP-PLA-NPs.
RESULTS AND CONCLUSION: The INH-RFP-PLA-NPs were smooth and uniform without obvious adhesion. The submicron particle size analyzer showed the mean diameter was 80.4 nm. The drug loading and entrapment efficiency of isoniazid were (15.95±1.34)% and (5.01±0.17)% resectively, and those of rifampicin were (4.66±0.97)% and (4.05±0.18)% respectively. Release in vitro of INH-RFP-PLA-NPs was stable. Release rate of isoniazid was 15.22% in burst release phrase, and rose to 95.6% after 3 days; release rate of rifampicin was 9.26% and rose to 90.3% after 3 days. INH-RFP-PLA-NPs prepared by modified spontaneous binary solvent diffusion method have small and uniform mean diameter. Release in vitro of INH-RFP-PLA-NPs is stable without obvious burst release.

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