Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (16): 2865-2868.doi: 10.3969/j.issn.1673-8225.2012.16.004

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Preparation and characterization of an integrative poly (hydroxybutyrate-co-hydroxyoctanoate) osteochondral tissue-engineering scaffold

Guo Xiao-fei1, Zhang Yong-hong2, Du Mei-li1, Lü Li-hua1, Chen Xue-ying3, Zhao Liang-qi1   

  1. 1Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan  03006, Shanxi Province, China; 2Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan  03001, Shanxi Province, China; 3Shanxi Tissue Bank, Taiyuan  03001, Shanxi Province, China
  • Received:2011-11-15 Revised:2012-02-23 Online:2012-04-15 Published:2012-04-15
  • Contact: author: Zhao Liang-qi, Doctoral supervisor, Professor, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 03006, Shanxi Province, China liangqi@ sxu.edu.cn
  • About author:Guo Xiao-fei★, Studying for master’s degree, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 03006, Shanxi Province, China sx_xiaofei@126.com
  • Supported by:

    the National Natural Science Foundation of China, No. 31040030*

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Abstract:

BACKGROUND: Single-layer scaffold, however, is difficult to meet the requirements of articular cartilage injury and repair. The integrative scaffold makes up partial defects of a single-layer scaffold.
OBJECTIVE: To prepare an integrative scaffold made of poly (hydroxybutyrate-co-hydroxyoctanoate)-hydroxyapatite to repair articular cartilage and subchondral bone defects.
METHODS: The scaffold was prepared by solvent casting/particle leaching method, and its aperture, porosity, mechanical strength and cell adhesion growth were tested.
RESULTS AND CONCLUSION: The optimal ratio of porogenic agent to scaffold material was 4.5/1 for the cartilage layer, 2.5/1 for the middle layer, and 3.5/1 for the bone layer. The porosity and pore size of the scaffold were 75% and 150-450 μm in the bone layer, 84% and 150-250 μm in the cartilage layer, 60% and ≤60 μm in the middle layer, respectively. The layers linked to be a whole one closely. The mechanical strength was 4.3, 6.43, and 2.93 MPa from the bone layer to the cartilage layer. The scaffold displayed good affinity to the cells. The technique of integrative scaffold made of poly (hydroxybutyrate-co-hydroxyoctanoate)-hydroxyapatite w as developed. The scaffold may be a competent candidate in application in articular cartilage damage repair.

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