Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (15): 2720-2723.doi: 10.3969/j.issn.1673-8225.2012.15.013

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三七总皂苷对L6大鼠成肌细胞抗化学损伤模型的保护作用**

Yang Yan-hua, Zhu Dao-li, Chen Pei-lin, Yu Chun-mei, Yang Yu-qin   

  1. School of Life Science, Nantong University, Nantong  226109, Jiangsu Province, China
  • Received:2011-09-15 Revised:2011-12-20 Online:2012-04-08 Published:2012-04-08
  • Contact: author: Zhu Dao-li, Professor, School of Life Science, Nantong University, Nantong 226109, Jiangsu Province, China zhudaoli@ntu.edu.cn
  • About author:Yang Yan-hua, School of Life Science, Nantong University, Nantong 226109, Jiangsu Province, China
  • Supported by:

    Innovative Training Project for Students of Nantong University, No. 2010038*; Special Foundation for Doctor Station of Nantong University, No. 05024276*

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Abstract:

BACKGROUND: Panax notoginseng saponin has multiple pharmacological effects such as stasis and clearing and activating the channels and collaterals, but the mechanisms are unclear.
OBJECTIVE: To observe the protective effect of panax notoginseng saponin against H2O2 damage of L6 rat myoblasts and its effect on the expression of Bax and bcl-2.
METHODS: H2O2 damaged model of L6 rat myoblasts was built. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, 4,6-diamino-2-phenyl indole chemical fluorescence and flow cytometry were applied to detect the survival rates of L6 rat myoblasts treated with different dosesof panax notoginseng saponin (0.5 , 0.1 and 0.02 g/L). The effects of different doses of panax notoginseng saponin (0.5, 0.1 and 0.02 g/L) on Bax and Bcl-2 protein expression levels of L6 rat myoblasts were analyzed by immunofluorescence cytochemistry.
RESULTS AND CONCLUSION: The L6 rat myoblasts had low survival rate and high apoptosis rate after injury of H2O2, while panax notoginseng saponin could obviously increase the survival rate and reduce the apoptosis rate. Immunofluorescence cytochemistry results showed that panax notoginseng saponin could enhance the Bcl-2 expression and inhibit the Bax expression. Panax notoginseng saponin can protect L6 rat myoblasts from H2O2 damage, and the protective effects are in order of sequence of 0.1 g/L > 0.02 g/L >     0.5 g/L . The mechanism may be related to the enhancement of Bcl-2 expression and inhibition of Bax expression.
Yang YH, Zhu DL, Chen PL, Yu CM, Yang YQ. Protective effect of panax notoginseng saponin against chemical damage of myoblasts in L6 rats. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2720-2723.    
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