Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (15): 2724-2727.doi: 10.3969/j.issn.1673-8225.2012.15.014

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Changes in ischemic skeletal muscle of the limbs in diabetic rats after multi-point injection of recombinant plasmids carrying hepatocyte growth factors into the skeletal muscles around the femoral arteries

Hu Chao-chang1, 2, Ha Xiao-qin2, He Yu-mei3, Xie Fu-qiang4, Xing Zhan-kui4, Han Jun-ping5   

  1. 1School of Stomatology, Lanzhou University, Lanzhou  730000, Gansu Province, China; 2Experimental Center of Medicine, General Hospital of Lanzhou Military Area Command, Lanzhou  730050, Gansu Province, China; 3Baiyin Hospital of Integrated Traditional Chinese and Western Medicine, Baiyin  730900, Gansu Province, China; 4Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Lanzhou University, Lanzhou  730010, Gansu Province, China; 5Institute of Pathology, Lanzhou University, Lanzhou  730000, Gansu Province, China
  • Received:2011-10-08 Revised:2011-11-11 Online:2012-04-08 Published:2012-04-08
  • Contact: Xie Fu-qiang, Professor, Master’s supervisor, Department of Oral and Maxillofacial Surgery, the Second Affiliated Hospital of Lanzhou University, Lanzhou 730010, Gansu Province, China Xiefq@lzu.edu.cn Ha Xiao-qin, Doctor, Chief physician, Master’s supervisor, Experimental Center of Medicine, General Hospital of Lanzhou Military Area Command, Lanzhou 730050, Gansu Province, China haxq@yahoo.com
  • About author:Hu Chao-chang★, Studying for master’s degree, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China; Experimental Center of Medicine, General Hospital of Lanzhou Military Area Command, Lanzhou 730050, Gansu Province, China hcc0602@yahoo.cn
  • Supported by:

    Gansu Provincial Technology and Research Project, No. 1011FKCA103*

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Abstract:

BACKGROUND: At present, plasmid vectors are widely used in gene therapy based on their better bio-security and a longer duration in vivo.
OBJECTIVE: To investigate the histological changes of ischemic skeletal muscle of the hind limbs in diabetic rats after multi-point injection of recombinant plasmids carrying hepatocyte growth factors (pUDKH) into muscles around the femoral arteries for 15 days.
METHODS: Streptozotocin was used to establish diabetes model in Sprague-Dawley rats, and then the rats were divided into three groups randomly. After 24 hours of modeling, rats in the high dosage group were injected with pUDKH at 200 μg for each, those in the low dosage group were injected with pUDKH at 100 μg for each, and those in the control group were treated with medical water for injection at the same volume. Skeletal muscles of the rats were removed for pathological observation at 15 days.
RESULTS AND CONCLUSION: Sarcoplasmic atrophy and degeneration, fibrotic hyperplasia and hyalinization, invasive degeneration of plasma cells, widened muscle space, and focal abscess could be seen in the control group. Plentiful capillaries were formed after the treatment by pUDKH, and stripes of muscle fibers were well kept in the high dosage group compared with the low dosage group. It is indicated that pUDKH carrying hepatocyte growth factors has the therapeutic effects on Limb ischemia of diabetic rats.
Hu CC, Ha XQ, He YM, Xie FQ, Xing ZK, Han JP. Changes in ischemic skeletal muscle of the limbs in diabetic rats after multi-point injection of recombinant plasmids carrying hepatocyte growth factors into the skeletal muscles around the femoral arteries. Zhongguo Zuzhi Gongcheng Yanjiu. 2012;16(15): 2724-2727.     [http://www.crter.cn  http://en.zglckf.com]

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